Recombinant Human Transforming Growth Factor-Beta 3, CHO
Synonyms Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3.
Introduction Transforming growth factor betas (TGFBetas) mediate many cell-cell interactions that occur during embryonic development. Three TGFBetas have been identified in mammals. TGFBeta1, TGFBeta2 and TGFBeta3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule.
Recombinant Human Transforming Growth Factor-Beta 3, CHODescription TGF-b 3 Human Recombinant produced in CHO is a disulfide-linked homodimeric, glycosylated, polypeptide chain containing 112 amino acids and having a molecular mass of 25kDa.
The TGF-b 3 is purified by standard chromatographic techniques.
Source Chinese Hamster Ovarian Cells.
Physical Appearance Sterile Filtered clear solution.
Recombinant Human Transforming Growth Factor-Beta 3, CHOFormulation The protein solution contains 20% Ethanol and 0.1% Acetic acid.
Stability TGF-beta 3 although stable at 4℃. for 3 weeks, should be stored at -20℃ to -70℃.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Purity Greater than 97.0% as determined by SDS-PAGE.
Amino acid sequence The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Leu-Asp-Thr-Asn.
Biological Activity The biological activity was determined using cell toxicity assay via HT-2 cells. The ED50 was found to be < 0.05ng/ml, corresponding to a specific activity of 20,000,000IU/mg.
Recombinant Human Transforming Growth Factor-Beta 3, CHOUsage CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.