Recombinant Human Interleukin-27
Synonyms Interleukin-30, IL-30, IL27/p28, p28, Interleukin-27, Interleukin-27/p28, IL27, Interleukin-27 subunit alpha, IL27 subunit alpha, IL27-A, Il27, Il27a, IL27p28.
Introduction Interleukin-27 protein is related to IL-12A & is one of the subunits of a heterodimeric cytokine complex. IL27 interacts with EBV induced gene 3 also called EBI3, and forms a complex that drives rapid expansion of CD4 (+) T cells.
IL27 complex is synergizes with IL-12 in order to trigger the cytokine production of IFN-Gamma of CD4 (+) T cells. The biological effect of IL27 is mediated by class-I cytokine receptor (WSX1/TCRR).
The pro-inflammatory activity of IL27 is mediated through the growing expression of key molecules involved in the MHC class-I & MHC class-II pathways. Both MHC class-I and MHC-class-II expression are increased in endothelial cells after Interleukin-27 stimulation which suggests that it may play an important role in conferring the immune function on vascular endothelium IL27p28 subunit can be induced by IFN-beta and during LPS-induced maturation of dendritic cells in type-I IFN-dependent manner through IFN regulatory factor-1 activation.
Interleukin-27 regulates Interleukin-12 responsiveness of CD4+ T cells through Stat1-dependent and -independent mechanisms. IL17 & IL-23 play an important IL17 role in inflammation. Interleukin-27 possesses potent anti-angiogenic activity that plays an important role in its antitumor and antimetastatic activities.
EBV induced gene 3 plays a role, independently from IL27, in regulating anti-viral or anti-tumoral immune responses. Interleukin-27 is a potent inhibitor of HIV-1 replication in macrophages, CD4+ T cells, peripheral blood mononuclear cells.
Interleukin-27 triggers STAT activation and gene transcription.
Recombinant Human Interleukin-27Description Interleukin-27 Human Recombinant produced in HEK 293 is a glycosylated non-covalently linked heterodimer, consisting of one p28 subunit (215 amino acids) and one EBI3 subunit (209 amino acids), with a total a molecular weight of 47.8kDa.
Human IL27 is purified by proprietary chromatographic techniques.
Source HEK 293 cells
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from 10mM Na2PO4 + 50mM NaCl, pH 7.5.
Recombinant Human Interleukin-27Solubility It is recommended to reconstitute the lyophilized Interleukin-27 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized Interleukin-27 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL27 should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
Purity Greater than 95.0% as determined by
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(c) UV spectroscopy at 280 nm.
Amino acid sequence EBI3: RKGPPAALTL PRVQCRASRY PIAVDCSWTL PPAPNSTSPV SFIATYRLGM AARGHSWPCL QQTPTSTSCT ITDVQLFSMA PYVLNVTAVH PWGSSSSFVP FITEHIIKPD PPEGVRLSPL AERQLQVQWE PPGSWPFPEI FSLKYWIRYK RQGAARFHRV GPIEATSFIL RAVRRRARYY VQVAAQDLTD YGEWSLP ATATMSLGK
P28: FPRPPGRPQL SLQELRREFT VSLHLARKLL SEVRGQAHRF AESHLPGVNL YLLPLGEQLP DVSLTFQAWR RPERLCF ISTTLQPFHA LLGGLGTQGR WTNMERMQLW AMRLDLRDLQ RHLRFQVLAA GFNLPEEEEE EEEEEEEERK GLLPGALGSA LQGPAQVSWP QLLSTYRLLH SLELVLSRAV RELLLLSKAG HSVWPLGFPT LSPQP
Recombinant Human Interleukin-27Biological Activity The activity is determined by its ability to inhibit IL-2 production from activated mouse spleenocytes and is typically 60-90ng/mL.
Usage CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.