- ¥2900
- solarbio
- 中国
- H7980
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8℃
- 保质期：
1年
- 英文名：
High Oxidized LDL
- 库存：
准现货
- 供应商：
solarbio
- CAS号：
H7980
- 规格：
2mg
HIGH OXIDIZED LOW DENSITY LIPOPROTEIN, HUMAN
Catalog No: H7990
Quantity: 2.0mg(miligrams) Protein/vial
Concentration: 2.0mg/ml
Packaging: High Oxidized LDL is membrane filtered and aseptically packaged in a solution containing phosphate-buffered saline at pH 7.4.
Preparation: Human LDL (which was purified to homogeneity via ultra centrifugation(1.019-1.063g/cc)) is extensively oxidized with Cu2SO4 (oxidant) in PBS at 37°C. Oxidation is terminated by adding excess EDTA-Na2. Each lot is analyzed on agarose gel electrophoresis for migration versus LDL. This lot of OxLDL migrates 3.0fold further than the native LDL. This product can produce potent oxidative stress and can be used to induce cell apoptosis/death(>50%) and produce cell injure model.
TBARS: TBARS is determined colorimetrically by using Malondialdehyde as a standard.
Starting LDL 0.12 nmoles of MDA/mg Protein
High Ox-LDL 91.6 nmoles of MDA/mg Protein
Storage: his product is stable for only 4 weeks after receipt when handled aseptically and stored at 2-8°C. NEVER FREEZE AND PROTECT FROM LIGHT.
Special Note: After prolonged storage, some precipitate may be observed. This is normal for this product. Clarify out the aggregates by spinning in a microfuge for 2 minutes.
Biological: Sample lots of Oxidized LDL are evaluated for receptor binding to
Activity: peritoneal macrophages in conjunction with our DiI-Ox-LDL and [I-125] Ox-LDL
*Preparations of High Oxidized LDL are fairly unstable; Plan your experiments in advance and use fresh materials.
FOR RESEARCH USE ONLY
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文献和实验Oxidized LDL and Lp(a): Preparation, Modification, and Analysis
with oxidized LDL described in the literature are achieved with differing preparations, ranging from minimally modified to extensively oxidized LDL. It is therefore the purpose of this chapter to describe methods for the preparation and the oxidative
Oxidized Low-Density Lipoprotein
Oxidized low-density lipoprotein (Ox-LDL) has been studied for over 25 years. Numerous pro- and anti-atherogenic properties have been attributed to Ox-LDL. Yet, Ox-LDL has neither been defined nor characterized, as its components
Determination of Acrolein by High-Voltage Capillary Electrophoresis from Oxidized Fatty Acids
from fatty acid oxidation, although it is particularly important because it constitutes the major electrophyle aldehyde derived from lipid oxidation. Acrolein can be formed actively from oxidized fatty acids and undergo aldolic condensation in alkaline pH
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