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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Delta Like Protein 4 (dLL4)
- 库存:
1000
- 供应商:
上海信裕
| Organism species | Rattus norvegicus (Rat) |
| Product No. | xy256Ra01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 14.3kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Gly215~Ser331 with N-terminal His-Tag |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative. |
GKYCDQ PICLSGCHEQ NGYCSKPDEC NCRPGWQGPL CNECIPHNGC RHGTCTIPWQ CACDEGWGGL FCDQDLNYCT HHSPCKNGST CSNSGPRGYT CTCLPGYTGE HCELELSKCA S
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
δ样蛋白4(δLL4)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验“小众”的一类T细胞 我们常说的T细胞其实是指αβ T细胞,占T细胞群体的65%-70%,其表面的T细胞受体(TCRs)由两条糖蛋白链组成,α链和β链。而γδ T细胞是T细胞的一个亚群,占所有T淋巴细胞的0.5%-5%,其TCRs由γ链和δ链组成,主要存在于上皮和粘膜组织,比如皮肤、肠道等[2]。与传统的αβ T细胞不同,γδ T细胞能识别其目标抗原而不受MHC与限制,并介导抗肿瘤反应,而不会引起移植物抗宿主病(GvHD)[3]。 γδ T细胞是异质性的T细胞,根据其TCRs组成和细胞
网络 二、CD3γ、δ和 ε链 CD3ε、γ和δ链的cDNA序列分析表明,它们都属于I型跨膜蛋白,都含有一个约有50氨基酸残基组成的类似免疫球蛋白胞外功能域,编码这些肽链的基因密切连锁,可能起源于同一祖先基因。在复合体中,CD3γ、δ和ε以二种非共价键形式γε和δε异源二聚体存在。 ε链分子量为20-25kDa,从编码ε cDNA推算出多肽
应用锚定PCR技术对TCRδ链进行分析 【操作方法】 (1) 提取外周血淋巴细胞总RNA; (2) 利用oligo-dT或特异的C区序列作引物,在逆转录酶作用下合成第一条cDNA链; (3) 于cDNA 3’末端添加Poly(dG)尾反应液(含2mmol/L CoCl2 ,1 mmol/L dGTP)和脱氧核酸末端转移酶(TdT),37℃×1h;70℃终止反应,用乙醇沉淀DNA; (4) PCR扩增:基因5’端使用与J区序列配对的特异
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