- ¥1 - 3980
- PEPROTECH
- 中国/美国
- xy-880Ra01
- 2025年12月02日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Secreted Frizzled Related Protein 1 (SFRP1)
- 库存：
1000
- 供应商：
上海信裕
| Organism species | Rattus norvegicus (Rat) |
| Product No. | xy880Ra01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 18.2kDa |
| Concentration | 9.1 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
MGHHHHHHSG SEF- VMQ FFGFYWPEML KCDKFPEGDV CIAMTPPNAT EASKPQGTTV CPPCDNELKS EAIIEHLCAS EFALRMKIKE VKKENGDKKI VPKKKKPLKL GPIKKKELKR LVLFLKNGAD CPCHQLDNLS HNFLIMGRKV KSQYLLTAIH KWD
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
分泌型卷曲相关蛋白1(SFRP1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
. Cell 124 , 147–159. 4. Rodriguez, J., Esteve, P., Weinl, C., Ruiz,J.M., Fermin, Y., Trousse, F., et al. (2005)SFRP1 regulates the growth of retinal ganglioncell axons through the Fz2 receptor. Nat. Neurosci. 8 , 1301–1309. 5. Hoang, B., Moos, M
时限短暂；瞬时表达系统的优点是简捷，实验周期短。大规模的瞬时表达技术是 近年来的一个研究热点。已有报道能放大到100 L反应器中生产重组蛋白，产量(分泌型蛋白)可达l~10 mg/L。不过该方法技术条件要求高，如质粒的纯度、转染的效率等。 稳定表达系统是指载体进入宿主细胞并经选择培养，载体DNA稳定存在于细胞内，目的蛋白的表达持久、稳定。由于需抗性选择甚至加压扩增等步骤，稳定表达相对耗时耗力。Geisse等以白血病抑制因子的制备为例，比较了几种不同稳定表达体系在产量及时间上的差异(表 3)，把目的
降低其结合力。这一结果证明Hp在胃粘膜上皮细胞上的受体含有墨角藻糖(fucose)。而墨角藻糖结合的血型抗原，典型地在RBC上发现，也在人上皮细胞表面表达。Boren等为了弄清墨角藻糖酶敏感的Hp受体结构，他们比较了sc-IgA与S-IgA分子含有的不同墨角藻糖碳水化合物链的棋盘分析（Panel analysis）。最后他们证实了在人胃粘膜表面的Hp受体含有RBc Lewis分型的Leb抗原。他们还证实了Leb抗原是分泌型体质个体胃上皮细胞表面表达的最显著的血型相关抗原，而Lea 抗原是非分泌型体质个体
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