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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Uncoupling Protein 2, Mitochondrial (UCP2)
- 库存:
1000
- 供应商:
上海信裕
| Organism species | Rattus norvegicus (Rat) |
| Product No. | xy586Ra01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 14.2kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
MGHHHHHHSGSEF-LY DSVKQFYTKG SEHAGIGSRL LAGSTTGALA VAVAQPTDVV KVRFQAQARA GGGRRYQSTV EAYKTIAREE GIRGLWKGTS PNVARNAIVN CTELVTYDLI KDTLLKANLM TDDLPC
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
线粒体解偶联蛋白2(UCP2)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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指南根据问题的类型主要分成以下几类(以下资料权作参考,请勿盲目模仿!):1. 参考书推荐A. 对初学者看什么资料比较好?解答:《抗体技术实验指南》和Antibodies(a laboratory manual, wrote by Ed Harlow ,david lane)两本书不错。2. 针对样品的常见问题B. 做线粒体膜UCP2蛋白的Western Blot (以下简写成Western Blot),提取线粒体后冻存(未加蛋白酶抑制剂),用的博士德的一抗,开始还有点痕迹,现在越来越差,上样
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