- ¥1 - 3980
- PEPROTECH
- 中国/美国
- xy-020Ra01
- 2025年08月26日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant S100 Calcium Binding Protein A4 (S100A4)
- 库存：
1000
- 供应商：
上海信裕
| Organism species | Rattus norvegicus (Rat) |
| Product No. | xy020Ra01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | n/a |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
S100钙结合蛋白A4(S100A4)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验Characterization of Epithelial Cells in the Hair Follicle With S100 Proteins
proteins are expressed in very restricted regions of the hair follicle: S100A3 and S100A6 in distinct postmitotic differentiated epithelial cells and S100A4 and S100A6 in the epithelial stem cell compartments. Characterization of epithelial cells
这些差异可将蛋白从混合物如大肠杆菌裂解物中提取出来得到重组蛋白。蛋白的纯化大致分为粗分离阶段和精细纯化阶段二个阶段。粗分离阶段主要将目的蛋白和其他细胞成分如DNA、RNA等分开，由于此时样本体积大、成分杂，要求所用的树脂高容量、高流速，颗粒大、粒径分布宽.并可以迅速将蛋白与污染物分开，防止目的蛋白被降解。精细纯化阶段则需要更高的分辨率，此阶段是要把目的蛋白与那些大小及理化性质接近的蛋白区分开来，要用更小的树脂颗粒以提高分辨率，常用离子交换柱和疏水柱，应用时要综合考虑树脂的选择性和柱效两个因素。选择性指
、Glu、Ser和Thr的区域，且在该区域附近有某些特定的氨基酸，则该蛋白就会不稳定。这些PEST结构域的磷酸化导致钙的结合能力提高，从而利于钙依赖性蛋白酶对蛋白质的降解。这提示可以在缺乏PEST蛋白裂解系统的E.coli中表达PEST富含蛋白。 减少E.coli中重组蛋白裂解的策略有以下几种：（1）将蛋白质靶向细胞周质或培养基[145，186]；（2）在较低的温度下培养细菌[187]；（3）选用蛋白酶缺陷的菌株[188]；（4）构建N-末端或C-末端融合蛋白[186]；（5）将目的基因多拷贝串联
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