- ￥3000 - 3999
- Abbiotec
- 美国
- 250378
- 2026年03月14日
- E, WB
- Mouse
- H
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Denaturated HSA isolated from human plasma
- 亚型：
Mouse IgG1/k
- 形态：
Each vial contains 0.2 mg IgG in 0.2 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-A/G affinity chromatography.
- 保存条件：
Store at 4°C.
- 克隆性：
单克隆抗体
- 标记物：
见下方详细说明
- 适应物种：
H
- 宿主：
Mouse
- 应用范围：
E, WB
- 浓度：
见详细说明信息
- 靶点：
Albumin denatured (5D5)
- 抗体英文名：
Albumin denatured (5D5) Antibody
- 抗体名：
Albumin denatured (5D5)抗体
- 规格：
0.2 mg
Albumin denatured (5D5) Antibody产品详细介绍：
| 产品名称： | Albumin denatured (5D5) Antibody |
| 说明书： | http://abbiotec.com/antibodies/albumin-denatured-%285d5%29-antibody【参考图片】 |
| 货号： | 250378 |
| 规格： | 0.2 mg |
| 产品描述： | Human serum albumin (HSA) consists of a single polypeptide chain of 584 aminoacids, stabilized by 17 disulfide bridges. HSA is the most common protein in serum, it is produced in the liver and the concentration in serum is 35-50 mg/ml. HSA is often used as a carrier molecule because of its binding capacity, and thus mainly functions as the regulator of the colloidal osmotic pressure of the blood. The molecular weight is 67.5 kDa. This antibody is specific for denatured HSA. |
| 克隆类型： | Mouse Monoclonal Antibody |
| 亚型： | Mouse IgG1/k |
| 免疫原： | Denaturated HSA isolated from human plasma |
| 适用物种： | H |
| 应用范围： | E, WB |
| 应用说明： | Product Cat. No. 250378 reacts strongly in ELISA with denatured HSA directly coated onto the microtiterwell. Product Cat. No. 250378 reacts in western blot with denatured HSA in non-reduced form only. E: 1:5,000; WB: 1:1,000 |
| 别名： | Serum albumin; ALB |
| 形态： | Each vial contains 0.2 mg IgG in 0.2 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-A/G affinity chromatography. |
| 保存条件： | Store at 4°C. Product is guaranteed 6 months from the date of shipment. |
| 参考文献： |
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文献和实验environment with little denaturation. Purified antibodies: Once purified, if the antibody is at less than 0.1 mg/ml add a small amount of detergent or protein (0.1% TWEEN-20 or 1% bovine serum albumin) and some protease inhibitors to the solution
volumes running buffer (the serum albumin should stick to the column and the Ig should flow through).(6) On ice slowly add saturated ammonium sulfate to 45% (550 ml sample 450 ml saturated ammonium sulfate). Tilt overnight at 4°C.(7) Pellet by spinning
Antibody Purification This protocol includes an ammonium sulfate cut, affigel blue chromatography and affinity chromatography. Solutions Affigel Blue Prewash 0.1 M acetic acid 5.7 ml glacial acetic acid
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