- ¥1 - 3980
- PEPROTECH
- 中国/美国
- xy-290Ra01
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC)
- 库存：
1000
- 供应商：
上海信裕
| Organism species | Rattus norvegicus (Rat) |
| Product No. | xy290Ra01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | n/a |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
V-Myc骨髓细胞瘤病毒癌基因同源物(MYC)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验摘要 Groundbreaking work demonstrated that ectopic expression of four transcription factors, Oct4, Klf4, Sox2, and c-Myc, could reprogram murine somatic cells to induced pluripotent stem cells (iPSCs) (Takahashi and Yamanaka, 2006), and human
肿瘤都发现了能导致细胞恶性转化的核酸片段，称之为癌基因。来自病毒的称为病毒癌基因（v-onc），来自细胞的称为细胞癌基因（c-onc）或原癌基因（proto-oncogene），它们具有转化的潜能，可被激活成为癌基因。后来发现从 酵母 菌到人类的正常细胞几乎都有与之类似的片段。这种进化上的高度保守性表明它们具有重要的生物学意义。 最早发现的病毒癌基因是鸡Rous肉瘤病毒的v-src基因，以后又发现了与之同源的、见于正常鸡细胞的细胞癌基因（c-src
crk,SH2/SH3调节蛋白 vav ,SH2调节蛋白 胞核蛋白 转录因子 myc myb fos jun erb A,T3受体 从上表可以看出，所有细胞癌基因均在信号转导中扮演着重要角色。正因为如此，它们的异常或“变质”--表达失控或表达产物异常--对细胞的危害就很大，既然v-onc只是c-onc的一个复本，那么为什么c-onc本不致癌而v-onc又会致癌呢？实验证明，v-onc的致癌或由于表达的失控，或由于基因的突变，导致产物
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