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- PEPROTECH
- 中国/美国
- xy-253Ra01
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Prolylcarboxypeptidase (PRCP)
- 库存：
1000
- 供应商：
上海信裕
脯氨酰羧肽酶(PRCP)重组蛋白
Recombinant Prolylcarboxypeptidase (PRCP)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Rattus norvegicus (Rat) |
| Product No. | xy253Ra01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 30.5kDa |
| Concentration | 8.5 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
脯氨酰羧肽酶(PRCP)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MLVFAEHR YYGKSLPFGR DSFKDSQHLN FLTSEQALAD FAELIRHLKE TIPGTEGQPV IAIGGSYGGM LAAWFRMKYP HIVVGALAAS APIWQLDGMA PCGEFMKIVT KDFSQSGPHC SESIRRSWSV INRTGSG LQWLRDTLHL CSPLTSEKMP TLKGWIAETW VNLAMVNYPY ACNFLQPLPA WPIKEVCRYL RNPNVSDTVL LQNIFQALNV YYNYSGQTKC LNISQTTTSS
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MLVFAEHR YYGKSLPFGR DSFKDSQHLN FLTSEQALAD FAELIRHLKE TIPGTEGQPV IAIGGSYGGM LAAWFRMKYP HIVVGALAAS APIWQLDGMA PCGEFMKIVT KDFSQSGPHC SESIRRSWSV INRTGSG LQWLRDTLHL CSPLTSEKMP TLKGWIAETW VNLAMVNYPY ACNFLQPLPA WPIKEVCRYL RNPNVSDTVL LQNIFQALNV YYNYSGQTKC LNISQTTTSS
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
脯氨酰羧肽酶(PRCP)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
脯氨酰羧肽酶(PRCP)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
脯氨酰羧肽酶(PRCP)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
：直接进样 HPLC 检测 雅心生物多年来致力于重组蛋白酶的研究，可提供生物医药用酶及相应的申报用资料。测序级肽图用酶如重组胰蛋白酶、重组羧肽酶 B、V8 和重组糜蛋白酶等。
:1。 孵育温度及时间：37℃ 反应 30 分钟。 检测：直接进样 HPLC 检测。 雅心生物多年来致力于重组蛋白酶的研究，可提供生物医药用酶及相应的申报用资料。测序级肽图用酶如重组胰蛋白酶、重组羧肽酶 B、V8 和重组糜蛋白酶等。
和Fc段分离。同时蛋白A琼脂糖凝胶FF也可用于血清中IgG的分离，得到不含抗体的血清，蛋白A琼脂糖凝胶FF有很好的稳定性，能耐受37度3-5天而对柱子的性能没有影响，是分离抗体的最佳选择。 疏水色谱色谱填料苯基琼脂糖凝胶FF以及DEAE琼脂糖凝胶FF也场用于抗体的分离，只是特异性不如重组蛋白A琼脂糖凝胶FF好。 纯化IgG抗原最有效的办法是免疫亲和，具体的方法是把抗抗原的IgG直接偶联到环氧活化琼脂糖凝胶FF上，然后直接高pH吸附，低pH洗脱就可以得到需要的抗原。 对于高亲和力的抗体
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脯氨酰羧肽酶(PRCP)重组蛋白
¥1 - 3980
