- 询价
- PEPROTECH
- 中国/美国
- xy-855Ra01
- 2025年07月14日
14 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)
- 库存：
1000
- 供应商：
上海信裕
核因子κB受体激活因子配基(RANκL)重组蛋白
Recombinant Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Rattus norvegicus (Rat) |
| Product No. | xy855Ra01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 19.0kDa |
| Concentration | 7.9 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
核因子κB受体激活因子配基(RANκL)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ESEDT EALPDSCRRM KQAFQGAVQR ELQHIVGPQR FSGVPAMMEG SWLDVARRGK PEAQPFAHLT INAADIPSGS HKVSLSSWYH DRGWAKISNM TLSNGKLRVN QDGFYYLYAN ICFRHHETSG SVPADYLQLM
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ESEDT EALPDSCRRM KQAFQGAVQR ELQHIVGPQR FSGVPAMMEG SWLDVARRGK PEAQPFAHLT INAADIPSGS HKVSLSSWYH DRGWAKISNM TLSNGKLRVN QDGFYYLYAN ICFRHHETSG SVPADYLQLM
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
核因子κB受体激活因子配基(RANκL)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
核因子κB受体激活因子配基(RANκL)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
核因子κB受体激活因子配基(RANκL)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
L? Protein A—只与 Fc 区域结合 Protein G—结合 Fc 区域和部分轻链 Protein A/G—重组蛋白，兼具 Protein A 和 Protein G 的结合位点 Protein L—结合仅限于那些含有 κ 轻链的抗体 省事且通用可选择 Protein A/G，也可根据结合力表格进行选择，详见资源信息部分 3、基质—琼脂糖还是磁珠？ 琼脂糖 磁珠 优点 简单易用，无需辅助设备 操作简单 孔径大，直径大（50-150
抗体的亚型。市场上还有一种重组的Protein A和G的重组蛋白偶联的填料，即Protein A/G，它综合了二者的优点，使纯化过程更加方便。 另一个问题是IgM亚型的单抗并不是总是可以用Protein A来纯化的，一个比较好的办法是采用另一种亲和填料来纯化:Protein L resin，其原理和Protein A和G不太一样，Protein L可以特异性地结合抗体的Kappa链。在小鼠体内，至少95%的抗体是κ型的（另5%是λ型的），所以绝大部份单抗都可以用Protein L亲和填料纯化，其中就包括
技术资料暂无技术资料 索取技术资料
核因子κB受体激活因子配基(RANκL)重组蛋白
询价
