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- PEPROTECH
- 中国/美国
- xy-667Ra01
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Glucokinase (GCK)
- 库存：
1000
- 供应商：
上海信裕
葡萄糖激酶(GCK)重组蛋白
Recombinant Glucokinase (GCK)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Rattus norvegicus (Rat) |
| Product No. | xy667Ra01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | n/a |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
葡萄糖激酶(GCK)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
葡萄糖激酶(GCK)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
葡萄糖激酶(GCK)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
葡萄糖激酶(GCK)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验相关实验
耐量异常。PPAR- 激动剂 WY14、643治疗可纠正MKR小鼠糖代谢异常。（二）MODY2动物模型zhang等利用基因敲除技术制得肝脏葡萄糖激酶(GCK) -小鼠，发现随着时间延长，该鼠血糖逐渐升高，糖耐量减退，6周龄小鼠空腹血糖显著高于对照组小鼠，这种肝细胞GCK活性减退所引起的疾病与人MODY相似，可作为MODY的动物模型。（三）线粒体糖尿病Silva等发现β细胞Tfam(mitochondrial transcriptionfactor A)突变的小鼠，大约5周龄时发生糖尿病，表现为严重mtDNA耗竭
明显糖耐量异常；PPAR- 激动剂 WY14,643治疗可纠正MKR小鼠糖代谢异常。 2、MODY2动物模型 zhang等利用基因敲除技术制得肝脏葡萄糖激酶（GCK） -/-小鼠，发现随着时间延长，该鼠血糖逐渐升高，糖耐量减退，6周龄小鼠空腹血糖显著高于对照组小鼠，这种肝细胞GCK活性减退所引起的疾病与人MODY相似，可作为MODY的动物模型。 3、线粒体糖尿病 Silva等发现β细胞Tfam（mitochondrial transcriptionfactor A）突变的小鼠，大约5周龄时发生
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葡萄糖激酶(GCK)重组蛋白
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