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DYKDDDDK Tag An
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DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #2368
3
上海恒斐生物科技有限公司
现货
40ul
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry)
DYKDDDDK Tag Antibody detects exogenously expressed DYKDDDDK proteins in cells. The antibody recognizes the DYKDDDDK peptide (the same epitope recognized by Sigma's Anti-FLAG® antibodies) fused to either the amino- or carboxy-terminus of targeted proteins. The binding specificity of this antibody is NOT dependent on the presence of divalent metal cations.
DYKDDDDK标签抗体(生物素化) 可以检测细胞中外源性表达DYKDDDDK的蛋白。此抗体可以识别与目的蛋白氨基末端或羧基末端的融合的DYKDDDDK肽段(与Sigma公司的抗FLAG抗体识别相同的表位)。此种抗体结合的特异性不依赖于二价金属离子的存在。
Polyclonal antibodies are produced by immunizing animals with a synthetic DYKDDDDK peptide. Antibodies are purified by protein A and peptide affinity chromatography.
多克隆抗体是采用合成的DYKDDDDK肽段免疫动物而生产的(KLH-coupled) 。抗体采用蛋白A和亲和色谱法纯化。
Western blot analysis of extracts from COS or 293T cells, mock transfected (lane 1) or expressing DYKDDDDK-tagged p73 (lane2), DYKDDDDK-tagged FKHRL1 (lane 3) or truncated DYKDDDDK-tagged huntingtin (lane 4), using DYKDDDDK Tag Antibody.Western blot 分析COS和293T细胞的提取物,泳道1为mock转染组,泳道2为采用DYKDDDDK--tagged p73转染组,泳道3为DYKDDDDK-tagged FKHRL1 转染组,泳道4为truncated DYKDDDDK-tagged huntingtin转染组。所用抗体为DYKDDDDK Tag Antibody 。
Confocal immunofluorescent analysis of COS cells, untransfected (right) or transfected with a construct overexpressing DYKDDDDK-tagged FKHRL1 (left) using DYKDDDDK Tag Antibody.激光共聚焦免疫荧光分析COS细胞,右图为未转染组,左图为过表达DYKDDDDK-tagged FKHRL1 的构建体的转染组,所用抗体为DYKDDDDK Tag Antibody
Immunohistochemical analysis of paraffin-embedded COS cells, control (left) or DYKDDDDK-FKHRL1 transfected (right), using DYKDDDDK Tag Antibody.免疫组化分析石蜡包埋的COS细胞,左图为对照组,右图为DYKDDDDK-FKHRL1 转染组。所用抗体为DYKDDDDK Tag Antibody。
Flow cytometric analysis of DYKDDDDK Tag Rabbit antibody in Cos cells transfected with a Myc-DDK-tagged SUMF1 construct and gated on transfected (green) or untransfected (blue) cells using Myc-Tag (9B11) Mouse mAb (Alexa Fluor® Conjugate) #2279.
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
在免疫印迹、免疫沉淀和免疫染色等技术中,表位标签用于标记和检测蛋白。由于它们的体积很小,它们一般不会影响标记蛋白的生化特性。
The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (1).
DYKDDDDK肽段作为常规的表位标签被广泛应用于表达载体中,此肽段可以通过与目的蛋白的氨基末端或羧基末端融合而被表达和检测(1)。
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