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文献和实验Mag-Binds Plant DNA Kit Magnetic 96 Protocol
isolation protocol. Mechanical tissue disruption: Place sample into a stainless steel grinding jar with appropriate steel beads. Freeze samples in the stainless steel grinding jar using liquid nitrogen for 1 minute. Immediately attach
Analysis of Gene Expression in Bone by Quantitative RT-PCR
(2 ). In this procedure, the tissue is powdered in a stainless steel ball mill vessel that is cooled in liquid nitrogen. We have used a ball mill and modified Trispin method to extract high-quality, RT-PCR-ready RNA from bone samples.
Preparation and Staining of Frozen Tissue Sections
is easily exposed when sections are cut. Place a stainless steel beaker of 2-methylbutane in liquid nitrogen and allow to cool adequately. Place base mold with tissue into the beaker of cold 2-methylbutane and quickly immerse the block. Allow
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