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文献和实验Large Scale Tubulin Preparation
. Hold the flask in a warm water-filled sink and swirl constantly to dissolve the solids and to mix in the glycerol. Transfer to a 37¡C bath, monitor temperature of mixture using a clean thermometer and polymerize for 60' after the temperature
Large Scale Tubulin Preparation
-filled cooler to the lab within 1-2 hours of removal. For transporting 10-12 brains from the slaughterhouse, we use 2 size 16 Coleman coolers containing 3 liters of cooled 1.5% (w/v) NaCl to which one large bag of party ice is added on the way
Large Scale Tubulin Preparation
-filled cooler to the lab within 1-2 hours of removal. For transporting 10-12 brains from the slaughterhouse, we use 2 size 16 Coleman coolers containing 3 liters of cooled 1.5% (w/v) NaCl to which one large bag of party ice is added on the way
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