Human 5-hydroxytryptamine (serotonin) receptor HTR1A (wild type) cloned into pcDNA3.1+ (Invitrogen) at BamHI (5') and XhoI (3'). The open reading frame was amplified by the PCR fromHuman whole brain cDNA (Clontech). The insert was sequenced. Insert size = 1270 bp.
N-terminal 3xHA-tagged Human 5-hydroxytryptamine (serotonin) receptor HTR1A (wild type) cloned into pcDNA3.1+ (Invitrogen) at KpnI (5') and XhoI (3'). The open reading frame was derived from HTR01A0000 by PCR. The insert was sequenced. Insert size= 1354bp.
5-hydroxytryptamine (serotonin) receptor (HTR1B) (wild type) cloned into pcDNA3.1+ (Invitrogen) at EcoRI (5') and XhoI (3'). The open reading frame was amplified by the PCR from Human Genomic DNA. The insert was sequenced. Insert size= 1173 bp
N-terminal 3xHA-tagged human 5-hydroxytryptamine (serotonin) receptor 1B (HTR1B) cloned into pcDNA3.1+ (Invitrogen) at KpnI (5') and XhoI (3'). The open reading frame was derived from HTR01B0000 by PCR. The insert was sequenced. Insert size= 1257bp.
Human 5-hydroxytryptamine (serotonin) receptor HTR1D (wild type) cloned into pcDNA3.1+ (Invitrogen) at EcoRI (5') and XbaI (3'). The open reading frame was amplified by the PCR from Image clone (4299633). The insert was sequenced. Insert size = 1140 bp.