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Magna ChIRP
上海恒斐生物科技有限公司
12 Assays
Description | |
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Catalogue Number | 17-10494 |
Trade Name |
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Description | Magna ChIRP RNA Interactome Kit -Isolation and characterization of non-coding RNA:chromatin complexes |
Overview | Features and Advantages
The Magna ChIRP RNA Interactome Kit uses the ChIRP method (Chromatin Isolation by RNA Purification) to enable identification and analysis of regions of chromatin that interact with a particular chromatin-associated RNA. This novel anti-sense probe-based capture strategy uses cross-linked chromatin to allow unbiased discovery of RNA-associated DNA sequences and proteins. To perform ChIRP two pools consisting of mulitple biotinylated oligonucleotide probes complementary to the RNA of interest are used (see figure 1). These probe sets are combined with chromatin and hybridized to the chromatin-associated RNA. Complexes containing biotinylated-probes bound to the chromatin-associated RNA are then isolated using streptavidin magnetic beads. DNA or RNA can then be recovered and analyzed by quantitative PCR or next generation sequencing (ChIRP-seq). Alternatively, proteomic analysis of the recovered chromatin can be performed. Learn more about the ChIRP method in JOVE! (Watch Now!) Need help making your own ChIRP probe sets? (Use the ChIRP probe designer tool.) |
Packaging Information | |
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Material Size | 12 Assays |
Material Package | Kit capacity: 12 Chromatin Isolation by RNA Purification Assays (includes negative control probes) |
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