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【简单介绍】
INCELL’s M3™ Medium is a complex formulation with multiple growth factors and supplements.【详细说明】
INCELL’s M3™ Medium is a complex formulation with multiple growth factors and supplements. M3™ was formulated to grow fragile and fastidious human and animal stem cells from multiple types of normal and diseased tissues.
M3TM Base Culture Media
Description: M3:Base™ is a proprietary, serum free, highly enriched culture media for fastidious human and mammalian cell types. M3:Base™contains numerous growth factors, supplements, and buffers, but may require blood components or serum additives, depending on cell type. M3:Base™is used as a basal medium and contains antibiotics* unless otherwise indicated.
Item # |
Unit Type |
Unit Size |
Unit Price |
M300A-100 |
Media (A) |
100 ml* |
|
M300A-500 |
Media (A) |
500 ml |
|
M300A-500 |
Media (A) |
Case of 6/500 |
|
M3TM Base F Culture Media
Description: M3:F Base™is a proprietary, serum free, highly enriched culture media for fastidious human and mammalian cell types. M3:F Base™contains numerous growth factors, supplements, and buffers, but may require blood components or serum additives, depending on cell type. M3:F Base™is used as a basal medium and is antibiotic free (F). Antibiotics can be added.
Item # |
Unit Type |
Unit Size |
Unit Price |
M300-F100 |
Media (F) |
100 ml* |
|
M300F-500 |
Media (F) |
500 ml |
|
M300F-500 |
Media (F) |
Case of 6/500 ml |
|
M3DTM Defined Media
Description :M3:D™is a defined medium used as the starting solution for all M3 Media. M3D™ contains salts, amino acids, and sugars, but no growth factors or undefined components such as serum or extracts.
Item # |
Unit Type |
Unit Size |
Unit Price |
M3DEF-100 |
Media (F) |
100 ml* |
|
M3DEF-500 |
Media (F) |
500 ml |
|
M3DEF-500 |
Media (F) |
Case of 6/500 ml |
|
M3:10TM Culture Media
Description: M3:10™, a derivative of M3™base, contains a proprietary formulation, growth factors, 10% Fetal Bovine Serum (FBS) and antibiotics* unless otherwise specified. M3:10™ is used to grow embryonic and adult stem cells, fastidious mesenchymal cells as attached cultures and as the "starter medium" for most tumor cells (attached and/or suspension cultures). Useful with many standard cell lines to improve growth and expression of differentiation and/or organotypic genes.
Item # |
Unit Type |
Unit Size |
Unit Price |
M310A-100 |
Media (A) |
100 ml* |
|
M310A-500 |
Media (A) |
500 ml |
|
M310A-500 |
Media (A) |
Case of 6/500 ml |
|
Media Designations:
(A): Contains Antibiotics
(F): Antibiotics-Free
(G): Contains Gentamycin
(S): Custom Formulation
* Limited Quantities Available
To Order: Please call 1.800.364.1765
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文献和实验We use two different kinds of media. Most cells are grown in DMEM. A few lymphoid cell lines are grown in RPMI. Cells grown in DMEM must be grown in a 10% CO2 atmosphere. As a result, most of our incubators are set for this. In contrast, cells grown
Raft culture media (aka Green’s media)
Raft culture media (aka Green’s media) 3 parts DMEM (including glutamine or glutamax) 1 part Ham’s F12 5% FCS various supplements (not everybody uses the same supplements, some don’t use
Cell Culture Media and Solutions
gentamicin reagent Filter sterilize through a 0.22 � cellulose acetate filter and store up to 2 weeks at 4 degrees C. Wash media: (1 liter) 10.0 ml 2.5 M (100X) Hepes buffer 1.2 ml 50 mg/ml gentamicin reagent Filter sterilize
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