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文献和实验PARP1 Genomics: Chromatin Immunoprecipitation Approach Using Anti-PARP1 Antibody (ChIP and ChIP-seq)
cycle, they may both act as epigenetic markers. However, it is not known how many loci are occupied by PARP1 protein during mitosis genome-wide. To reveal the genome-wide PARP1 binding sites, we used the ChIP-seq approach, an emerging technique to study
Chromatin Immunoprecipitation (ChIP)
gel Antibodies to native protein of interest (or a post-translationally modified form) Antibody (control) Cell lysis buffer for ChIP (cold) Cells (50-100 million per experiment) Dilution buffer EDTA Elution
).8. Block with 4% BSA in TBST for 45 - 60 minutes. I use RIA grade BSA from USB (United States Biochemical; Catalog number 10868). I also include 0.02% NaN3 in this block and reuse it at least 5 times (Keep in 4oC between uses). We have had problems
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