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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
1年以上
- 英文名:
Brilliant Cresyl Blue
- 库存:
大量
- 供应商:
solarbio
- CAS号:
81029-05-2
英文名:Brilliant Cresyl Blue
别名:煌焦油蓝;亮焦油兰
CAS:81029-05-2
货号:B8820
规格:10g
分子式:C34H42Cl4N8O2Zn
分子量:801.95
用途:生化研究,活体染色剂(指示氧化还原电位),血液染色(检查血小板和网状细胞),氧化还原指示剂(蓝色~无色)。棉布印花等。
储存:RT
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文献和实验% polyacrylamide gel containing blue-stained beta-casein (0.5 mg/ml)(Novex). After electrophoresis was complete, the gel was incubated for 1 hour at 25℃ in a 2.5% Triton X-100 solution, washed two times, 20 minutes each, with water and then incubated overnight
Analysis of Proteins using Small Format 2D Gel Electrophoresis
dithiothreitol, 2.3% (w/v) SDS) for 7 minutes before embedding in 1% (w/v) agarose prepared in 0.125M Tris-HCl, pH 6.7, 0.1% (w/v) SDS with bromophenol blue to colour. The second dimension slab gels (70 x 80 mm) are prepared in batches of 12 gels using
ul 1M DTT (from frozen stock) or 40 ul 2-ME solution.Loading sample buffer with bromophenol blue20 ml 1 x SDS sample buffer2 ml bromophenol blue (1mg/ml) 0.19% in DDW. Aliquot 1ml and store 4¡C.10x SDS running buffer pH ~8.3 (2 Lt total): Trizma base60
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