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- 文献和实验
- 技术资料
- 保存条件：
常温干燥保存
- 保质期：
十二个月(详询)
- 库存：
100
- 供应商：
远慕生物
- 规格：
g/kg/mg
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文献和实验earth. After RNase treatment, the DNA containing supernatant is bound to diatomaceous earth in a chaotropic buffer of guanidine hydrochloride by incubation at room temperature. The DNA-associated diatomaceous earth then is collected by centrifugation
蛋白复合体直接酶消化法Direct Enzymatic Digestion of Protein Complexes
protocols. The methods presented here include a double digest with endoproteinase Lys-C and trypsin, or a triple digest with elastase, subtilisin, and trypsin. Endoproteinase Lys-C cleaves carboxy-terminal to lysine residues, while trypsin cleaves carboxy
Determination of Protein Contacts by Chemical Cross‐Linking With EDC and Mass Spectrometry
and the resulting peptides recovered. Chemically bonded peptides are distinct from the multitude of unmodified single peptides by the presence of two carboxy?terminal ends. The protocol also describes incorporation of 18 O at the carboxy?terminal ends
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