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- 详细信息
- 文献和实验
- 技术资料
- 靶点:
来电咨询
- 浓度:
1mg/1ml
- 应用范围:
科研使用
- 宿主:
Goat,Rabbit,Mouse
- 适应物种:
人,大鼠,小鼠,兔
- 标记物:
见说明书
- 克隆性:
多克隆
- 保存条件:
-20℃保存
- 形态:
液态/粉状
抗体的相关标记:HRP,Biotin,Gold,RBITC,AP,FITC,Cy3,Cy5,Cy5.5,Cy7,PE,PE-Cy3,PE-Cy5,PE-Cy5.5,PE-Cy7,APC,Alexa Fluor 350,Alexa Fluor 488,Alexa Fluor 555,Alexa Fluor 647
ChromPure Goat IgG, whole molecule - Agarose的交叉反应:人,小鼠,大鼠,鸡 ,狗,猪,羊,牛,兔等......
二抗
产品目录号。
批号和参考数据。
稀释度。
稀释液。
孵育的时长。
孵育的温度。
ChromPure Goat IgG, whole molecule - Agarose已经确定的最佳条件,节省您宝贵的时间,样品和试剂。包括:
最优的稀释和缓冲液
已指定的阳性和阴性对照细胞提取物
已经优化的详细的操作步骤
ChromPure Goat IgG, whole molecule - Agarose在一些如胸腺、脾脏、血液的组织以及造血细胞、淋巴细胞、B细胞等细胞内,通常含有较多的Fc受体,这时候选择二抗时最好选择Anti-IgG, F(ab’)2 fragment这样的特异性二抗,这样就消除了由于Fc部分与IgG的非特异性结合。常规的Anti-IgG (H+L)二抗与IgG的重链和轻链都有结合反应,即与IgG的Fc的F(ab’)2片段都能结合;由于IgG、IgM、IgA都有保守的轻链结构域,因此Anti-IgG (H+L)与它们都有交叉反应。而Anti-IgG, F(ab’)2 fragment经过了IgG Fc片段的吸附,因此只与IgG的F(ab’)2片段结合,然而IgG、IgM、IgA都有保守的轻链结构域,因此与它们也有交叉反应。
ChromPure Goat IgG, whole molecule - Agarose:xy-0573xy Cyclin E周期素E/原癌基因抗原
xy-0497xy Dnmt3a(cytosine-5)-methyltransferase 3A)DNA甲基转移酶-3α(抗原)
xy-0282xy Cyclin C周期素 C(抗原)
xy-0791xy CGRP (calcitonin gene related peptide, CGRP)降钙素基因相关肽
xy-0614xy CULLIN (Cdc53/CUL)
xy-0461xy CYP450(Cytochrome P450 monooxygenase)细胞色素P450单氧化酶(多肽抗原)
xy-0488xy CXorf36(uncharacterized protein Cxorf36 precursor)脱羧酶蛋白体36 cxorf36抗原
xy-0399xy Deltex-1(DTX1) (Deltex protein 1)抗原
xy-0428xy DISC1(disrupted in schizophrenia1)(NT)DISC1 N端抗原
xy-0430xy DISC1(disrupted in schizophrenia1)(CT)DISC1 C端抗原
xy-0421xy DR3/TRAMP (death receptor-3)DR3 死亡受体(抗原)
xy-0714xy E7 protein/HPV 16 (Human papillomavirus type 16)人乳头瘤病毒16型E7蛋白抗原
xy-0165xy EGF-R (Epidemal growth factor receptor)表皮生长因子受体(抗原)
xy-0613xy Eukaryotic aspartyl protease(Rice)天冬氨酸蛋白酶家族蛋白(水稻蛋白的一种)(抗原)
xy-0711xy FAM3B/PANDER(PANcreatic DERived factor)一种新型的胰岛细胞因子(抗原)
xy-0788xy Fabp2 (fatty acid binding protein 2, intestinal)脂肪酸结合蛋白2(抗原)
xy-0500xy ECG2 (sophagus cancer-related gene-2)食道癌相关基因(抗原)
xy-0666xy Fibronectin (FN)纤维连结蛋白(多肽抗原)
xy-0734xy FGF-7/KGF (Keratinocyte growth factor precursor)成纤维细胞生长因子-7/纤维母细胞生长因子 (抗原)
xy-0675xy FGFR2(Fibroblast Growth Factor Receptor 2)成纤维细胞生长因子受体2抗原
xy-0735xy FGF-8/HBGF-8 (Fibroblast growth factor 8)成纤维细胞生长因子-8/纤维母细胞生长因子-8 (抗原)
xy-0400xy GAD-65 (glutamate decarboxylase)谷氨酸脱羧酶-65(C端多肽)
xy-0721xy Galectin-3 半乳糖凝集素-3(抗原)
xy-0701xy GCS (glucosylceramide synthase, GCS)葡萄糖神经酰胺合成酶(抗原)
xy-0639xy GFP绿色荧光蛋白
xy-0725xy ER-alpha雌激素受体alpha
xy-0654xy GHR (growth hormone receptor)生长激素受体(抗原)
xy-0472xy GLUT1(Glucose transporter type 1)葡萄糖转运蛋白1(抗原)
xy-0618xy hypothetical protein (LOC55652)hypothetical protein 抗原
xy-0402xy HCCR-1(BRI3 binding protein)人宫颈癌基因/bri3结合蛋白(抗原)
xy-0343xy pre S1/S2 protein [HBV]乙肝病毒pre S1/乙肝病毒pre S2(抗原)
xy-0728xy HCV-HCBP1/ACY-3(Hepatitis C virus core-binding protein 1)丙型肝炎病毒核心蛋白结合蛋白-1(抗原)
xy-0746xy HHV8/ORF50(Human herpesvirus 8 type P)人类疱疹病毒8抗原
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文献和实验ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
is available in which the albumin binding site has been deleted. Agarose beads coated with these proteins will bind antibodies and serve to immunoprecipitate antigens from solution. There are differences in the affinity of these proteins for antibodies
protein depends upon the species that the antibody was raised in. Protein A binds well to rabbit, cat, human, pig and guinea pig IgG as well as mouse IgG2a and IgG2b. Protein G binds strongly to IgG from cow, goat, sheep, cow, horse, rabbit and guinea pig
ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
strongly to IgG from cow, goat, sheep, cow, horse, rabbit and guinea pig and to mouse IgG1 and IgG3 . Protein G can also bind bovine serum albumin (BSA). Thus, BSA should be added to buffers used with Protein G. Alternatively, recombinant Protein G
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