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文献和实验Chromatin Immunoprecipitation Assays for Myc and N-Myc
Myc and N-Myc have widespread impacts on the chromatin state within cells, both in a gene-specific and genome-wide manner. Our laboratory uses functional genomic methods including chromatin immunoprecipitation (ChIP), ChIP-chip
Myc is a transcriptional coordinator of a wide range of intracellular and extracellular processes required for cell proliferation. These processes are tightly regulated in physiological conditions but hijacked when Myc is oncogenically
In this chapter, we discuss in detail two essential methods used to evaluate the interaction of Myc with another protein of interest: co-immunoprecipitation (Co-IP) and in vitro pull-down assays. Co-IP is a method that, by immunoaffinity
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