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- 详细信息
- 文献和实验
- 技术资料
- 浓度:
1mg/ml
- 应用范围:
科学研究
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Chicken,Dog,Cow,Rabbit,Sheep
- 标记物:
PE HRP Gold Cy5.5 Cy7 RBITC PE-CY5.5 PE-CY7 APC FITC Cy3 Cy245
- 克隆性:
Polyclonal
- 保存条件:
-20℃
- 形态:
液态/冻干粉
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived from human/mouse/Rat
- 规格:
7 ml
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文献和实验antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000 - 1:20,000 dilution in 4% BSA in TBST. This should be titrated for optimal results. I incubate with primary Ab 3 hours - overnight at room temp with shaking.10. Remove the antibody solution
% nonfat dry milk works well for anti-PCREB, but in my hands milk is not as good as BSA from USB (it seems to mask the epitope). Remove block solution and add primary antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000
. 3- add the AP conjugated secondary antibody (50 to 100 µl) and incubate for 45 min. The secondary antibody should be absorbed against human serum; if not add 1% human serum before use. SBA Goat anti mouse AP or Goat anti Rabbit AP can be used 1:200
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