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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
KLH conjugated synthetic peptide derived from human/mouse/Rat
- 亚型:
IgG
- 形态:
液态/冻干粉
- 保存条件:
-20℃
- 克隆性:
Polyclonal
- 标记物:
PE HRP Gold Cy5.5 Cy7 RBITC PE-CY5.5 PE-CY7 APC FITC Cy3 Cy1798
- 适应物种:
Human,Mouse,Rat,Chicken,Dog,Cow,Rabbit,Sheep
- 宿主:
Rabbit
- 应用范围:
科学研究
- 浓度:
1mg/ml
- 规格:
500 ug
抗体纯化/标记/免疫检测
免疫分析检测是一项应用广泛的技术,利用抗原和抗体的特异性反应进行检测的一种手段,广泛用于医学病理学、免疫组织化学、分子生物学、生物制药等领域的分析研究与技术测定。制备高特异性、高效价的抗体是实验免疫学技术的基础,抗体质量的高低将直接影响试验的成败。抗体纯化能降低非特异性本底,抗体标记能通过标记物的增强放大效应提高检测灵敏度,采用ELISA、WB、IP、IHC等不同免疫分析技术检测各种样品中特定的目的蛋白。 我公司拥有国内领先的抗体服务技术、先进的仪器设备和专业的抗体技术人员,可为客户提供:(1)多种抗体纯化服务,如硫酸铵纯化、Protein A/G纯化、抗原亲和纯化等,帮助客户解决各种抗体纯化问题;(2)一流的抗体标记服务,包括生物素标记、荧光素标记、酶标记等;(3)多种免疫检测分析 技术服务:酶联免疫吸附实验(ELISA),免疫印迹(WB),免疫沉淀(IP)和免疫组化(IHC)等。
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文献和实验secondary antibody review -- data from 99 publications
Signaling 18 IgG Alexa Fluor 488 immunocytochemistry 1:2000 18 IgG Alexa Fluor 568 immunocytochemistry 1:2000 19 donkey IgG HRP western blot 1:3000 detect antibody binding in mouse epithelial cells Amersham Biosciences 19 goat
E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
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