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- 详细信息
- 文献和实验
- 技术资料
- 靶点:
Myc
- 应用范围:
ChIP
- 适应物种:
H
- 克隆性:
多克隆
- 保存条件:
-20℃
- 规格:
500µl
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文献和实验PCR Primers For Gene Expression Detection or Quantification
: by GenBank Accession, NCBI protein accession, LocusLink ID, PrimerBank ID or Keyword (gene description). PrimerBank contains about 180,000 primers covering most known human and mouse genes. Polymerase Chain Reaction Amplification (PCR
SuperScript III One-Step RT-PCR System with Platinum® Taq High Fidelity
concentration of 0.2 µM for each primer is generally optimal. However, for best results, we recommend performing a primer titration of 0.15-0.5 µM. 8) Design primers that anneal to the mRNA sequence in exons on both sides of an intron
在RT-PCR中避免DNA污染(Avoiding DNA Contamination in R
-PCR primer choice with respect to minimizing the problems associated with DNA contamination is to design primers that span at least one intron of the genomic sequence. This will result in a PCR product from genomic contamination that will be larger in size
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