DBC1 Antibody

5693:

Immunofluorescence , Immunoprecipitation , Western Blotting

DBC1 Antibody detects endogenous levels of total DBC1 protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human DBC1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from various cell lines using DBC1 Antibody.

IF-IC

Confocal immunofluorescent analysis of HeLa cells using DBC1 Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red).

Deleted in breast cancer gene 1 protein (DBC1) was originally identified by its localization to a region of chromosome 8p21 that is homozygously deleted in breast cancer (1). DBC1 is a large, nuclear protein with multiple functions in cell survival. It binds directly to the estrogen receptor α (ERα) hormone-binding domain in a ligand-independent manner and may be a key determinant of ligand-independent ERα expression and survival in human breast cancer cells (2). DBC1 can promote p53-mediated apoptosis by binding to and inhibiting the deacetylase activity of SirT1, resulting in increased p53 acetylation levels and activity (3). DBC1 may be an important regulator of heterochromatin formation as it binds SUV39H1 and inhibits its histone methyltransferase activity (4). Caspase-dependent processing activates the pro-apoptotic activity of DBC1 during Tumor Necrosis Factor-α (TNF-α)-mediated cell death signaling (5). This processing of DBC1 in response to TNF-α is an early event in the onset of apoptosis and results in relocalization of DBC1 to the cytoplasm. Overexpression of the processed, cytoplasmic form of DBC1 results in mitochondrial clustering and matrix condensation and sensitizes cells to TNF-α-mediated apoptosis.

1. Hamaguchi, M. et al. (2002) Proc Natl Acad Sci USA 99, 13647-52.
2. Trauernicht, A.M. et al. (2007) Mol Endocrinol 21, 1526-36.
3. Zhao, W. et al. (2008) Nature 451, 587-90.
4. Li, Z. et al. (2009) J Biol Chem 284, 10361-6.
5. Sundararajan, R. et al. (2005) Oncogene 24, 4908-20.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

• 4880 Phospho-DBC1 (Thr454) Antibody
• 4526 Phospho-ATM (Ser1981) (10H11.E12) Mouse mAb
• 2873 ATM (D2E2) Rabbit mAb
• 2853 Phospho-ATR (Ser428) Antibody
• 2790 ATR Antibody
• 2851 Phospho-(Ser/Thr) ATM/ATR Substrate Antibody
• 2909 Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb
• 2493 SirT1 (D739) Antibody
• 2991 SUV39H1 Histone Methyltransferase Antibody
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 9998 BSA
• 9999 Nonfat Dry Milk

For Research Use Only. Not For Use In Diagnostic Procedures.