GAP43 Antibody

5307:

Western Blotting

GAP43 Antibody detects endogenous levels of total GAP protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GAP43 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from mouse and rat brain using GAP43 Antibody.

GAP43 is a nervous system specific, growth-associated protein enriched in growth cones and areas of high plasticity (1). Phosphorylation of GAP43 at Ser41 by PKC is regulated by intracellular Ca²⁺ and affects the ability of GAP43 to bind calmodulin (2,3). GAP43 is integral to growth cone formation, neurite outgrowth, and the development of a functional cerebral cortex (4,5). Aberrant GAP43 expression can be seen in patients diagnosed with schizophrenia and Alzheimer's disease (6,7).

1. Biewenga, J.E. et al. (1996) Acta Biochim Pol 43, 327-38.
2. Dekker, L.V. and Parker, P.J. (1997) J Biol Chem 272, 12747-53.
3. Chapman, E.R. et al. (1991) J Biol Chem 266, 207-13.
4. Aigner, L. and Caroni, P. (1993) J Cell Biol 123, 417-29.
5. Shen, Y. et al. (2008) Cerebellum 7, 451-66.
6. Sower, A.C. et al. (1995) Mol Chem Neuropathol 24, 1-11.
7. de la Monte, S.M. et al. (1995) Am J Pathol 147, 934-46.

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• 4830 Calmodulin Antibody
• 2261 Phospho-(Ser) PKC Substrate Antibody
• 2056 PKCα Antibody
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
• 9998 BSA
• 9999 Nonfat Dry Milk

For Research Use Only. Not For Use In Diagnostic Procedures.