- 询价
- Cell Signaling Technology
- 2079
- USA
- 2025年10月21日
- W, IP, IHC-P, IF-F
- Rabbit
- M
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- 详细信息
- 技术资料
- 抗体英文名：
Miwi (G82) Antibody
- 抗原：
synthetic peptide corresponding to the sequence surrounding Gly82 of mouse Miwi
- 应用范围：
W, IP, IHC-P, IF-F
- 宿主：
Rabbit
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 适应物种：
M
- 库存：
大量
- 供应商：
CST
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-F=Immunofluorescence (Frozen)
Reactivity Key: M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IHC-P IF-F | M | Endogenous | 90 | Rabbit |
| Protocols | |
|---|---|
| Specificity / Sensitivity | Miwi (G82) Antibody detects endogenous levels of total Miwi protein. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence surrounding Gly82 of mouse Miwi. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from various mouse tissues using Miwi (G82) Antibody (upper panel) or β-Actin Antibody (#4967) (lower panel). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse testis using Miwi (G82) Antibody. IF-F
Confocal immunofluorescent analysis of mouse testis using Miwi (G82) Antibody (green) and Pan-Keratin (C11) Mouse mAb #4545 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | The Drosophila piwi gene was identified as being required for the self-renewal of germline stem cells (1). Piwi homologs are well conserved among various species including Arabidopsis , C. elegans, and Homo sapiens (1). Both Miwi and Mili proteins are mouse homologs of Piwi and contain a C-terminal Piwi domain (2). Miwi and Mili bind to Piwi-interacting RNAs (piRNAs) in male germ cells and are essential for spermatogenesis in mice (3-5). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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