HSP40 Antibody

4868:

Flow , Immunofluorescence , Immunoprecipitation , Western Blotting

HSP40 Antibody detects endogenous levels of total HSP40 protein. This antibody does not cross-react with other HSPs.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to Glu300 of human HSP40/Hdj1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from HeLa, C2C12 and C6 cells, using HSP40 Antibody.

Flow Cytometry

Flow cytometric analysis of HeLa cells, using HSP40 Antibody (blue) compared to a nonspecific negative control antibody (red).

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or heat-treated (right), using HSP40 Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).

HSP40 and HSP40-like proteins represent a large family of chaperone proteins that are homologous to E. coli DnaJ protein (1). These proteins are classified into three subtypes based on their structures. The common feature of the family is a conserved J domain, which is usually located at the amino terminus of proteins and responsible for their association with HSP70 (1,2). Human HSP40, also known as Hdj1, belongs to subtype II that contain a unique Gly/Phe-rich region (2). HSP40 family proteins bind unfolded proteins, prevent their aggregation, and then deliver them to HSP70 (2,3). Another major function of HSP40 is to stimulate ATPase activity of HSP70, which causes conformational change of the unfolded proteins (4,5). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 or components of the protein degradation machinery such as CHIP and BAG-1, which either leads to protein folding or degradation, respectively (6).

1. Cheetham, M.E. and Caplan, A.J. (1998) Cell Stress Chaperones 3, 28-36.
2. Fan, C.Y. et al. (2003) Cell Stress Chaperones 8, 309-316.
3. Langer, T. et al. (1992) Nature 356, 683-689.
4. Liberek, K. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 2874-2878.
5. Cyr, D.M. et al. (1992) J. Biol. Chem. 267, 20927-20931.
6. Höhfeld, J. et al. (2001) EMBO Rep. 2, 885-890.

• da Silva Correia, J. et al. (2007) Proc Natl Acad Sci U S A 104, 6764-9. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

• 4356 HSF1 Antibody
• 2402 HSP27 (G31) Mouse mAb
• 4872 HSP70 Antibody
• 4873 HSP70 (6B3) Rat mAb
• 4874 HSP90 Antibody
• 4875 HSP90 (E289) Antibody
• 4876 HSP70 (D69) Antibody
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.