- 询价
- Cell Signaling Technology
- 4538
- USA
- 2026年05月28日
- W, IP, IHC-P, IF-IC, F
- H
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- 文献和实验
- 技术资料
- 抗体英文名：
MUC1 (VU4H5) Mouse mAb
- 抗原：
MUC1 60 mer tandem repeats (BSA conjugated)
- 应用范围：
W, IP, IHC-P, IF-IC, F
- 库存：
大量
- 供应商：
CST
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 适应物种：
H
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC F | H | Endogenous | 230, 400 | Mouse IgG1 |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | MUC1 (VU4H5) Mouse mAb detects endogenous levels of total MUC1. This antibody does not cross-react with other mucin proteins. |
| Source / Purification | Monoclonal antibody (isotype: IgG1k) is produced by immunizing a BALB/c mouse with MUC1 60 mer tandem repeats (BSA conjugated). Western Blotting
Western blot analysis of extracts from PL45 and MCF-7 cells using MUC1 (VU4H5) Mouse mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using MUC1 (VU4H5) Mouse mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MUC1 (VU4H5) Mouse mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using MUC1 (VU4H5) Mouse mAb. Flow Cytometry
Flow cytometric analysis of MCF-7 cells, using MUC1 (VU4H5) Mouse mAb (blue) compared to a nonspecifc negative control antibody (red). IF-IC
Confocal immunofluorescent analysis of MCF7 and MDA-MB-231 cells using MUC1 (VU4H5) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | Mucins represent a family of glycoproteins characterized by repeat domains and dense O-glycosylation (1). MUC1 (or mucin 1) is aberrently overexpressed in most human carcinomas. Increased expression of MUC1 in carcinomas reduces cell-cell and cell-ECM interactions. MUC1 is cleaved proteolytically, and the large ectodomain can remain associated with the small 25 kDa carboxy-terminal domain that contains a transmembrane segment and a cytoplasmic tail of 72 residues (1). MUC1 interacts with ErbB family receptors and potentiates ERK1/2 activation (2). MUC1 also interacts with beta-catenin, which is regulated by GSK-3beta, PKCdelta and Src through phosphorylation at Ser44, Thr41 and Tyr46 of MUC1 cytoplasmic tail (3-5). Overexpression of MUC1 potentiates transformation (6), and attenuates stress induced apoptosis through the Akt or p53 pathways (7,8).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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