- 询价
- Cell Signaling Technology
- 12656S
- USA
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 靶点：
For Kit 12656 Smad5 (Ser463/Ser465) phosphate/Smad5/Smad4/Smad1 (Ser463/Ser465) phosphate/Smad1/Smad1 (Ser206) phosphate
- 克隆性：
多克隆
- 保存条件：
-20℃
- 规格：
1Kit
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
SDS lysis buffer/1x proteinase inhibitor cocktail (2.5 µl of 200 x stock from Upstate EZ ChIP kit). Roughly dissociate heads by pipetting, and transfer them into a 2 ml dounce homogenizer. Total volume is ~700 µl. �SDS lysis
1 to a tube. 2) Add 20 μl of Antibody Mix. 3) Mix well and incubate for 20 min at 2–8°C. 4) Wash the cells by adding 2 ml Buffer 1. Mix well by tilting the tube several times and centrifuge at 300 x g for 10 min at 2–
All-in-One Kit for Immunohistochemical Staining for Tissues with Antibodies
O2 in methanol for 10 minutes. Wash slides 3 times with 1X PBS for 2 minutes, each time and then proceed to primary antibody incubation. 3. Epitope Retrieval Protocol A sample epitope retrieval protocol developed for some Zymed® Antibodies
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