Mouse TNF alpha ELISA Ready-SET-Go!® 10 x 96 tests w/plates产品说明书
Description
This Mouse TNF alpha ELISA Ready-SET-Go! reagent set (with or without high-affinity binding microwell plates)
contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent
assays (ELISA). This ELISA reagent set is specifically engineered for accurate and precise measurement of mouse
TNF alpha protein levels from samples including serum, plasma, tissue homogenate, lavage fluid and supernatants
from cell cultures. Recognition of mouse TNF alpha by this assay is not interfered with by 1000x excess soluble TNF
alpha receptor type I or II.
Components
Capture Antibody. Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating
Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part
10X Buffer into 9 parts dH20).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution
Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards
96 Well Plate. Corning Costar 9018 (included with product Cat. #’s ending in suffixes -22, -44, -76, -86)
References
Cho HJ, Seon MR, Lee YM, Kim J, Kim JK, Kim SG, Park JH. 3,3'-Diindolylmethane suppresses the inflammatory
response to lipopolysaccharide in murine macrophages. J Nutr. 2008 Jan;138(1):17-23. (RSG ELISA kit, TC
supernatant, PubMed)
Nakano T, Araki K, Nakatani H, Kobayashi M, Sugimoto T, Furuya Y, Matsuoka T, Jin T, Hanazaki K. Effects of
geldanamycin and thalidomide on the Th1/Th2 cytokine balance in mice subjected to operative trauma. Surgery