Rat TNFRSF1A (Tumor Necrosis F

Rat TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A) CLIA Kit本试剂盒采用双抗体夹心法。用抗Rat TNFRSF1A抗体包被于酶标板上，实验时标本或标准品中的TNFRSF1A会与包被抗体结合，游离的成分被洗去。依次加入生物素化的抗Rat TNFRSF1A抗体和辣根过氧化物酶标记的亲和素。抗Rat TNFRSF1A抗体与结合在包被抗体上的Rat TNFRSF1A结合、生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入发光底物混合液，发光底物在辣根过氧化物酶的催化下发出荧光，用化学发光免疫分析仪测定化学发光值（CL），TNFRSF1A浓度与化学发光值之间呈正相关，通过绘制标准曲线求出标本中TNFRSF1A的浓度。

英文名称	Rat TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A) CLIA Kit
中文名称	大鼠肿瘤坏死因子受体超家族成员1A(TNFRSF1A)化学发光免疫检测试剂盒
货号	YB-R0702c	种属	Rat/大鼠
规格	96T/Kit (8*12 strips) 48T/Kit (8*6 strips)
检测方法	双抗体夹心法
检测范围	15.625~1000pg/mL	灵敏度	9.375pg/mL

Rat TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A) CLIA Kit

中文名称	英文名称	规格	保存
CLIA酶标板	Micro CLIA Plate	8×12 / 8×6*	4℃/-20℃ #
冻干标准品	Reference Standard	2/1 支*	4℃/-20℃ #
标准品&样品稀释液	Reference Standard & Sample Diluent	1瓶 20mL/12mL*	4℃
浓缩生物素化抗体	Concentrated Biotinylated Detection Ab	1支 120μL/70μL*	4℃/-20℃ #
生物素化抗体稀释液	Biotinytated Detection Ab Diluent	1瓶 10mL/6mL*	4℃
浓缩HRP酶结合物	Concentrated HRP Conjugate	1支 120μL/70μL*	4℃(避光)
酶结合物稀释液	HRP Conjugate Diluent	1瓶 10mL/6mL*	4℃
浓缩洗涤液（25×）	Concentrated Wash Buffer (25×)	1瓶 30mL/16mL*	4℃
发光底物A液	Substrate Reagent A	1瓶 5mL/3mL*	4℃(避光)
发光底物B液	Substrate Reagent B	1瓶 5mL/3mL*	4℃(避光)
封板覆膜	Plate Sealer	5/3 张*
产品说明书	Manual	1 份
质检报告	Certificate of Analysis	1 份
特别说明： *: [96T/48T]（打开包装后请及时检查所有物品是否齐全完整） #: 一周内使用可存于4℃，需长时间存放或多次使用建议存于-20℃.

1.在各孔中加入标准品或样品各 100μL，37℃孵育90分钟
2. 倒去孔内液体，拍干，加入 100μL 生物素化抗体工作液，37℃孵育 60 分钟
3. 洗涤 3 次
4. 加入 100μL 酶结合物工作液， 37℃孵育 30 分钟
5. 洗涤 5 次
6. 加入 100μL 底物溶液， 37℃孵育 5 分钟左右
7. 读数
8. 结果计算
Rat TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A) CLIA Kit
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