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文献和实验Mag-Bind Blood RNA Isolation Protocol (for 50ul Blood)
1. Prepare a Lysis Mixture by mixing one volume of Buffer MRB and one volume of isopropanol. For 96 samples, combine15 ml Buffer MRB with 15 ml isopropanol and mix throughly by vortexing. Add 300 ul Lysis Mixture for each sample at step 3. 2. Add 50 ul
E.Z.N.A. Mag-Bind Blood DNA Protocol (1-100 ul Blood)
of liquid from the wall of the tube. 7. Remove the tube containing the Mag-Bind particles from the magnetic separation device. Add 300 ul Buffer MP/Ethanol mixture to each sample. 8. Resuspend the Mag-Bind particles pellet by vortexing at maxi
is cDNA concentration 200 ng/ul too high for qRT-PCR-Rea
The gene we are interested in looking at is not very abundant. At 100 ng/ul, the CT value is 30.9 In the literature that I have looked at, people seems to using much lower levels of cDNA. I would actually
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