DPABH-25028 is purified by affinity-chromatography using an epitode-specific phosphopetide. Non-phoso specific antibodies are removed by chromatography using a non-phosphopeptide.
Synthetic peptide corresponding to Human LIMK1. Synthetic peptide: around the phosphor site of Thr 508 (R-Y-T(p)-V-V) derived from Human LIMK1 (SwissProt: P53667) and of Thr 505 (R-Y-T(p)-V-V) derived from Human LIMK2 (SwissProt: P53671).Sequence: R-Y-T(p