- 询价
- R&D
- 美国/中国
- xy-580S
- 2025年07月12日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
14 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 形态：
液态/粉状
- 保存条件：
-20℃保存
- 克隆性：
多克隆
- 标记物：
见说明书
- 适应物种：
人，大鼠，小鼠，兔
- 宿主：
Goat，Rabbit，Mouse
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 靶点：
来电咨询
- GAPDH Monoclonal Antibody详细信息：
-
Description 
Monoclonal Antibody against Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH, G3PDH and GPDH) Clone 1D4 Form Tissue Culture Supernatant (contains 10mM Sodium Azide) Host Mouse Species Reactivity Mammalian including Human, Cow, Pig, Mouse, Rat and Avian IsoType GAPDH Monoclonal AntibodyIgG1, k light chain [Ab] ~1 mg/mL Specificity Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH as a protein expressed in large amounts and which is required at all times for an important "house keeping" functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimers disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntingtons disease. Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic.
This antibody was raised against extensively purified pig GAPDH.Uses This antibody is effective in immunoblotting, immunofluorescence (IF) and immuocytochemistry (ICC). Suggested Working Dilution GAPDH Monoclonal AntibodyThe optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. - Western blot: 1:1,000 or lower*
- IF: 1:100
Notes * Predicted MW = 38kD Storage Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. References Ellis RC, Earnhardt JN, Hayes RL, Wang KK, Anderson DK. Cathepsin B mRNA and protein expression following contusion spinal cord injury in rats. J. Neurochem. 88:689-697, 2004.
Fortun J, Dunn WA, Joy S, Li J, Notterpek L. Emerging role for autophagy in the removal of aggresomes in schwann cells. J. Neurosci. 23:10672-10680, 2003.
Dastoor Z, Dreyer J-L. Potential role of nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase in apoptosis and oxidative stress. J. Cell Sci. 114:1643-1653, 2001.
Burke JR, Enghild JJ, Martin ME, Jou Y-S, Myers RM, Roses AD, Vance JM, Strittmatter WJ. Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH. Nature Med. 2:347-350, 1996.
Schulze H, Schuler A, Stuber D, Dobeli H, Langern H, Huber G. Rat brain glyceraldehyde-3-phosphate dehydrogenase interacts with the recombinant cytoplasmic domain of Alzheimers beta-amyloid precursor protein. J. Neurochem. 60:1915-22, 1993.
Morgenegg G, Winkler GC, Hubscher U, Heizmann CW, Mous J, Kuenzle CC. Glyceraldehyde-3-phosphate dehydrogenase is a nonhistone protein and a possible activator of transcription in neurons. J. Neurochem. 47:54-62, 1986.Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - GAPDH Monoclonal Antibody
- xy-1401xy SRF(Serum response factor)血清应答因子抗原
xy-1402xy SREBP-1(Sterol Regulatory Element Binding Protein-1)固醇调节元件结合蛋白1抗原
xy-1381xy AKAP12A/SSeCKS peptide丝氨酸抑制蛋白激酶C底物抗原
xy-1702xy Fut4/CD15/SSEA-1(Stage-specific embryonic surface antigen 1)阶段特异性胚胎表面抗原-1抗原
xy-1137xy SSTR1 (somatostatin receptor 1)生长抑素受体1(SSTR1)抗原
xy-1138xy SSTR2 (somatostatin receptor 2)生长抑素受体2抗原
xy-1368xy SSTR3 peptide生长抑素受体3抗原
xy-1139xy SSTR5 (somatostatin receptor 5)生长抑素受体5抗原
xy-1317xy STAT1(signal transducers and activators of transcription 1)信号转导与转录激活因子1抗原
xy-1657xy phospho-STAT1(p-Tyr701) peptide磷酸化信号转导与转录激活因子1抗原
xy-1141xy STAT3(signal transducers and activators of transduction3)信号转导和转录激活因子3抗原
xy-1142xy STAT5(signal transducers and activators of transduction5)信号转导和转录激活因子5(STAT5)抗原
xy-1143xy STAT6(signal transducers and activators of transduction 6)信号转导和转录激活因子6抗原
xy-0629xy SULT1E1(Estrogen Sulfotranferase)雌激素硫酸转移酶抗原
xy-1499xy SUMO类泛素蛋白
xy-0885xy phospho-Tau protein (pThr489) peptide磷酸化微管相关蛋白多肽
xy-1369xy MAP-2(microtubule associated protein 2)微管相关蛋白2抗原
xy-1847xy MAP1A/Mtap1a(microtubule associated protein 1A)微管相关蛋白1A抗原
xy-1104xy TBX2/T-box2(T-box Protein 2)新型抑癌基因(多肽)
xy-1280xy TCF7L2(transcription factor 7-like 2)转录因子7类似物2抗原
xy-0822xy TDP-43/TARDBP (tar DNA binding protein)Tar DNA 结合蛋白43抗原
xy-1039xy Tenascin固生蛋白抗原
xy-2101xy TEM 1/CD248(Tumor endothelial marker 1 precursor)内涎蛋白/内皮唾液酸蛋白抗原
xy-0535xy TFF3 (trefoil factor3)三叶肽因子3(多肽)
xy-1144xy TFPI-2 (tissue factor pathway inhibitor-2)组织因子途径抑制剂-2抗原
xy-1838xy TIEG1/KLF10(TGF-beta inducible early response gene 1)TGF-β诱导早期应答基因1抗原
xy-0638xy TGF- BetaR1/ALK (TGF-beta receptor type I)转移生长因子–β受体1抗原
xy-1276xy Erdj5/DNAJC10Erdj5/DNAJC10多肽
xy-1334xy Tie1/JTK14 peptide上皮生长因子样域酪氨酸激酶1抗原
xy-1300xy Tie2(Porcine tyrosine kinase with immunoglobulin-like and EGF-like domains 2)上皮生长因子样域酪氨酸激酶2抗原
xy-1501xy Rarres2/TIG2/Chemerin(Retinoic acid receptor responder protein 2)视黄酸受体应答蛋白2抗原
xy-1444xy TLR3 (Toll-like receptor 3)Toll样受体3抗原
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
GAPDH抗体|GAPDH抗体大全-艾美捷指导您购买合适的GAPDH抗体
，但是进口品牌如Abcam、Millipore、CST等价格偏高，一般在￥3000/100ul左右。下面我们为您推荐三款GAPDH原装进口抗体，分别是美国EarthOx和ProteinTech的产品。EarthOx的Anti-GAPDH Monoclonal Antibody （货号E021010 ）是小鼠源的单抗，其效价很高，厂商推荐的WB起始稀释比率为1:1000，实际应用过程中，有使用1:10000或更高的报告，远远高于此稀释比率，说明其免疫效价很高，另外，其检测种属也很多，包括人、牛、猪、小鼠
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
技术资料暂无技术资料 索取技术资料
GAPDH Monoclonal Antibody
询价
