Polyclonal Antibody to Troponin I Type 3, Cardiac (TNNI3)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species
Mus musculus (Mouse)
Product No.
PAA478Mu01
Clonality
Polyclonal
Host
Rabbit
Immunoglobulin Type
IgG
Purification
Affinity Chromatography
Applications
WB, ICC, IHC-P, IHC-F, ELISA
Concentration
200ug/ml
UOM
100ug
Conjugate
No Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant TNNI3 (Met1~Gly211) expressed in E.coli.
ANTIBODY SPECIFITY
心肌肌钙蛋白I(TNNI3)多克隆抗体The antibody is a rabbit polyclonal antibody raised against TNNI3. It has been selected for its ability to recognize TNNI3 in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4,
containing 0.02% NaN3, 50% glycerol.
IHC Images
QUALITY CONTROL
Content: The quality control contains recombinant TNNI3 (Met1~Gly211) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
心肌肌钙蛋白I(TNNI3)多克隆抗体Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.