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- 详细信息
- 文献和实验
- 技术资料
- 形态:
液态/粉状
- 保存条件:
-20℃保存
- 克隆性:
多克隆
- 标记物:
见说明书
- 适应物种:
人,大鼠,小鼠,兔
- 宿主:
Goat,Rabbit,Mouse
- 应用范围:
科研使用
- 浓度:
1mg/1ml
- 靶点:
来电咨询
- Mouse Nestin Tail Polyclonal Antibody保存于运输说明:
- Store at -20°C
- 详细信息:
-
Description 
Polyclonal Antibody against Mouse Nestin Tail Clone 
Nestin 20 Form 
Antiserum Host 
Rabbit Species Reactivity 
Mouse Nestin Tail Polyclonal AntibodyHuman, mouse Specificity 
Nestin, a class IV intermediate filament protein is expressed during early stages of development in the CNS and in myogenic tissues. Upon differentiation, nestin becomes down regulated and is replaced by other tissue specific intermediate filaments. Antibodies against nestin can be used as a predominant marker to describe stem and neural progenitor cells in the mammalian CNS.
The polyclonal nestin antibody was raised using a KLH conjugated peptide to the mouse nestin tail. This antibody was tested using Ntera-2 cells and mouse embryonic cells and can be used for Western blotting and Immunofluorescence. In immunofluorescence in Ntera-2 and mouse embryonic cells, very beautiful filaments are detected with little background.Uses 
Mouse Nestin Tail Polyclonal AntibodyThis antibody is effective in immunoblotting, immunoflorescence and immunohistochemistry. Suggested Working Dilution 
The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. - Western blot: 1:1,000*
- Immunoflouresence: 1:200
- Immunohistochemistry: 1:500
Notes 
*Predicted MW= 220-240 kD Storage 
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. For long-term storage, keep antibody at -80°C. References 
Not Published Warranty/Conditions 
Covance products may not be resold or modified for resale without prior written approval. - Mouse Nestin Tail Polyclonal Antibody
- xy-0294Rs Rabbit anti-Goat IgG whole serum兔抗羊IgG抗血清
xy-0358M Mouse Anti-Guinea pig IgG小鼠抗豚鼠IgG
xy-0308Rs Rabbit anti-horse IgG whole serum兔抗马IgG抗血清
xy-0297M Mouse Anti-human IgG小鼠抗人IgG
xy-0374R Rabbit Anti-horse IgM兔抗马IgM
xy-0297G Goat Anti-human IgG羊抗人IgG
xy-0297R Rabbit Anti-human IgG兔抗人IgG
xy-0360R Rabbit Anti-human IgA兔抗人免疫球蛋白A
xy-0377R Rabbit Anti-mouse IgG-Fc兔抗小鼠IgG-Fc
xy-0309R Rabbit Anti-pig IgG兔抗猪IgG
xy-0309Rs Rabbit anti-Pig IgG whole serum兔抗猪IgG抗血清
xy-0293Rs Rabbit anti-Rat IgG whole serum兔抗大鼠IgG抗血清
xy-0293M Mouse Anti-rat IgG小鼠抗大鼠IgG
xy-0293R Rabbit Anti-rat IgG兔抗大鼠IgG
xy-0295M Mouse Anti-rabbit IgG小鼠抗兔IgG
xy-0296R Rabbit Anti-mouse IgG兔抗小鼠IgG
xy-0335R Rabbit Anti-Monkey IgG兔抗猴IgG
xy-0346R Rabbit Anti-rat IgM兔抗大鼠IgM
xy-0369M Mouse Anti-Rabbit IgM小鼠抗兔IgM
xy-0346Rs Rabbit anti-Rat IgM whole serum兔抗大鼠IgM抗血清
xy-0295Gs Goat anti-Rabbit IgG whole serum羊抗兔IgG抗血清
xy-0295xys Donkey anti-Rabbit IgG whole serum驴抗兔IgG抗血清
xy-0368G Goat Anti-Mouse IgM羊抗小鼠IgM
xy-0293G Goat Anti-rat IgG羊抗大鼠IgG
xy-0358G Goat Anti-Guinea pig IgG羊抗豚鼠IgG
xy-0345G Goat Anti-human IgM羊抗人IgM
xy-0294M Mouse Anti-Goat IgG小鼠抗羊IgG
xy-0312R Rabbit Anti-Avidin兔抗亲和素
xy-0311R Rabbit Anti-Biotin兔抗生物素抗体IgG
xy-0327R Rabbit Anti-Bovine IgM兔抗牛IgM
xy-0314R Rabbit Anti-chicken IgM兔抗鸡IgM
xy-0303R Rabbit Anti-dog IgG兔抗狗IgG
xy-0370R Rabbit Anti-Goat IgM兔抗羊IgM
xy-0358R Rabbit Anti-Guinea pig IgG兔抗豚鼠IgG
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文献和实验Polyclonal and Monoclonal Antibodies
Polyclonal Antibody Immunization of Mice: Injections should be made at intervals of at least two weeks.. We have been using either of the two following adjuvants with success: Freund adjuvant and MPL+TDM adjuvant ( RIBI ImmunoChem
Polyclonal and Monoclonal Antibodies(克隆和克隆抗体)
Adaped from David Bowtell (bowtell@ariel.ucs.unimelb.edu.au) Polyclonal Antibody Immunization of Mice: Injections should be made at intervals of at least two weeks.. We have been using
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
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