Polyclonal Antibody to Creatine Kinase, Muscle (CKM)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species
Canis familiaris; Canine (Dog)
Product No.
PAA109Ca01
Clonality
Polyclonal
Host
Rabbit
Immunoglobulin Type
IgG
Purification
Affinity Chromatography
Applications
WB, ICC, IHC-P, IHC-F, ELISA
Concentration
200ug/ml
UOM
100ug
Conjugate
No Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant CKM (Lys11~Leu367) expressed in E.coli.
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against CKM. It has been selected for its ability to recognize CKM in immunohistochemical staining and western blotting.
APPLICATIONS
肌肌酸激酶(CKM)多克隆抗体Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4,
containing 0.02% NaN3, 50% glycerol.
IHC Images
QUALITY CONTROL
Content: The quality control contains recombinant CKM (Lys11~Leu367) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
肌肌酸激酶(CKM)多克隆抗体Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.