Polyclonal Antibody to Alpha-2-Macroglobulin (a2M)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species
Homo sapiens (Human)
Product No.
PAB017Hu01
Clonality
Polyclonal
Host
Rabbit
Immunoglobulin Type
IgG
Purification
Affinity Chromatography
Applications
WB, ICC, IHC-P, IHC-F, ELISA
Concentration
200ug/ml
UOM
100ug
Conjugate
No Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant a2M (Ser616~Val856) expressed in E.coli.
ANTIBODY SPECIFITY
α2-巨球蛋白(α2M)多克隆抗体The antibody is a rabbit polyclonal antibody raised against a2M. It has been selected for its ability to recognize a2M in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4,
containing 0.02% NaN3, 50% glycerol.
IHC Images
QUALITY CONTROL
Content: The quality control contains recombinant a2M (Ser616~Val856) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
α2-巨球蛋白(α2M)多克隆抗体Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.