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- 详细信息
- 文献和实验
- 技术资料
- 形态:
液态/粉状
- 保存条件:
-20℃保存
- 克隆性:
多克隆
- 标记物:
见说明书
- 适应物种:
人,大鼠,小鼠,兔
- 宿主:
Goat,Rabbit,Mouse
- 应用范围:
科研使用
- 浓度:
1mg/1ml
- 靶点:
来电咨询
- p53 CM1 Polyclonal Antibody (SIGNET)详细信息:
-
Description 
Polyclonal Antibody against p53 Tumor Suppressor Gene Product
(Formerly Signet Catalog Nos: 520-01, 520-16 L1 Predilute, 520-26 L2 Predilute)Intended Use 
** In Vitro Diagnostic (IVD) ** Form 
Antiserum (diluted in 0.01M PBS + 0.1% NaN3 + 1% BSA) Host 
Rabbit Specificity 
p53 CM1 Polyclonal Antibody (SIGNET)The Signet anti p53 polyclonal antibody recognizes both wild type and mutant forms of the 53kDa oncoprotein.
The antibody was developed using recombinant p53 protein.
Concentrated Format:
SIG-3520-1000 [1 mL]
Prediluted Formats:
SIG-3520-16 [6 mL]
SIG-3520-26 [6 mL] is ready-to-use with Biotin-based detection systems such as Signet’s USA Ultrastreptavidin Detection (SIG-32250).Uses 
p53 CM1 Polyclonal Antibody (SIGNET)This antibody is effective in immunohistochemistry (IHC). Suggested Working Dilution 
The optimal working dilution should be determined for each specific assay condition. - IHC: ≥1:40 (concentrated format) for Biotin based detection systems such as Signet’s USA Ultrastreptavidin Detection (SIG-32250).
Tissue Sections: Formalin-fixed, paraffin-embedded tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Retrieve-All 1 (SIG-31910).
Incubation: 20-60 minutes at room temperature
Notes 
Positive tissue (human): Breast carcinoma Storage 
Store between 2-8°C. References 
Baas IO, Offerhaus JA, El-Deiry WS, Wu TC, Hutchins GM, Kasper EK, Braughman KL, Baumgartner WA, Chiou CJ, Hayward GS, Hruban RH; The WAF1-mediated p53 growth-suppressor pathway is intact in the coronary arteries of heart transplant recipients. Hum Pathol 27(4):324-9, 1996. Warranty/Conditions 
Covance products may not be resold or modified for resale without prior written approval. - IHC: ≥1:40 (concentrated format) for Biotin based detection systems such as Signet’s USA Ultrastreptavidin Detection (SIG-32250).
- p53 CM1 Polyclonal Antibody (SIGNET)
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xyE040Hu 围脂滴蛋白4(PLIN4)检测试剂盒 检测范围
xyE041Hu 围脂滴蛋白3(PLIN3)检测试剂盒 检测范围
xyE111Hu 肝配蛋白A5(EFNA5)检测试剂盒 检测范围
xyE175Hu 脂肪酶H(LIPH)检测试剂盒 检测范围
xyE211Hu 弹性蛋白酶3A(ELA3A)检测试剂盒 检测范围
xyE213Hu 糜蛋白酶原B1(CTRB1)检测试剂盒 检测范围
xyE214Hu 糜蛋白酶原B2(CTRB2)检测试剂盒 检测范围
xyE381Hu 钠/葡萄糖协同转运蛋白1(SGLT1)检测试剂盒 检测范围
xyE382Hu 钠/葡萄糖协同转运蛋白2(SGLT2)检测试剂盒 检测范围
xyE399Hu 甘氨酸转运蛋白1(GLYT1)检测试剂盒 检测范围
xyE459Hu 线粒体铁蛋白(MFRN)检测试剂盒 检测范围
xyE474Hu 溶质载体家族26成员4(SLC26A4)检测试剂盒 检测范围
xyE571Hu 卷曲同源物1(FZD1)检测试剂盒 检测范围
xyE671Hu SHC-转化蛋白1(SHC1)检测试剂盒 检测范围
xyE673Hu 羟甲基胆素合酶(HMBS)检测试剂盒 检测范围
xyE698Hu 鞘氨醇激酶1(SPHK1)检测试剂盒 检测范围
xyE711Hu 微管蛋白β3(TUBβ3)检测试剂盒 检测范围
xyE748Hu 亲核素α2(KPNα2)检测试剂盒 检测范围
xyE779Hu 膜联蛋白A9(ANXA9)检测试剂盒 检测范围
xyE780Hu 膜联蛋白A10(ANXA10)检测试剂盒 检测范围
xyE842Hu 白介素1ζ(IL1ζ)检测试剂盒 检测范围
xyE909Hu 组蛋白脱乙酰基酶9(HDAC9)检测试剂盒 检测范围
xyE913Hu 沉默调节蛋白3(SIRT3)检测试剂盒 检测范围
xyE915Hu 沉默调节蛋白5(SIRT5)检测试剂盒 检测范围
xyE917Hu 沉默调节蛋白7(SIRT7)检测试剂盒 检测范围
xyF196Hu 岩藻糖基转移酶6(FUT6)检测试剂盒 检测范围
xyF205Hu 腺苷酸激酶3(AK3)检测试剂盒 检测范围
xyF255Hu 钙蛋白酶6(CAPN6)检测试剂盒 检测范围
xyF264Hu 羰基还原酶3(CBR3)检测试剂盒 检测范围
xyF295Hu 封闭蛋白5(CLDN5)检测试剂盒 检测范围
xyF317Hu 羧肽酶A4(CPA4)检测试剂盒 检测范围
xyF387Hu 二肽酶3(DPEP3)检测试剂盒 检测范围
xyF396Hu 脊椎蛋白2(SPON2)检测试剂盒 检测范围
xyF540Hu 吻素受体(KISS1R)检测试剂盒 检测范围
xyF593Hu 多聚蛋白2(MMRN2)检测试剂盒 检测范围
xyF605Hu 黑素皮质激素5受体(MC5R)检测试剂盒 检测范围
xyF656Hu 磷酸二酯酶10A(PDE10A)检测试剂盒 检测范围
xyF714Hu 泛酸激酶4(PANK4)检测试剂盒 检测范围
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文献和实验of First Hospital of Harbin Medical University. RPMI 1640 was purchased from GIBCO. Propidium iodide and Rnase were from Sigma Chemical Co. A murine monoclonal antibody against human Bcl-2 and Bax oncoprotein and antimi ce rabbit polyclonal antibody were purchased
Studies of the Ubiquitin Proteasome System
) 6% to 8% SDS‐PAGE minigels (unit 6.1 ) Anti‐ubiquitin antibody ( protocol 31 ; for immunoblot detection) Boiling water bath Additional reagents and equipment for SDS
secondary antibody were purchased from GIBCO-BRL. Phosphospecific rabbit polyclonal antibodies against 180Thr and 182Tyr dual-phosphorylated p38, against total p38 (phosphorylation state independent), against 204Tyr-phosphorylated ERK1/2 and against total
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