The Signet polyclonal synaptophysin stains neuronal and adrenal tumors, and various neuroendocrine tumors of epithelial origin such as pancreatic islet cell, adenomas, pancreatic carcinomas and medullary carcinomas of the thyroid.
Synaptophysin (SYP) Polyclonal Antibody, Purified (SIGNET)This antibody was developed using synthetic synaptophysin sequence conjugated with PPD.
Concentrated Format: SIG-3261-1000 [1 mL]
Prediluted Format: SIG-3261-26 [6 mL] is ready-to-use with Biotin-based detection systems such as Signet’s USA Ultrastreptavidin Detection (SIG-32250).
Uses
This antibody is effective in immunohistochemistry (IHC).
Suggested Working Dilution
The optimal working dilution should be determined for each specific assay condition.
IHC: ≥1:40 (concentrated format) for Biotin based detection systems such as Signet’s USA Ultrastreptavidin Detection (SIG-32250). Tissue Sections: Formalin-fixed, paraffin-embedded tissues Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Retrieve-All 1 (SIG-31910). Incubation: 20-60 minutes at room temperature
Notes
Positive tissue (human): Pancreas
Storage
Synaptophysin (SYP) Polyclonal Antibody, Purified (SIGNET)Store between 2-8°C.
References
Chejfec G, Falkner S, Grimelius L, et al. Synaptophysin. A new marker for pancreatic neuroendocrine tumors. Am J Surg Pathol 11:241-7, 1987.
Navone F, Jahn R, Di Gioia G, Stukenbrok H, Grengard P, De Camilli P. Protein p38: an integral membrane protein specific for small vesicles of neurons and neuroendocrine cells. J Cell Biol 103:2511-27, 1986.
Wiedenmann B, Franke WW. Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles.Cell 41:1017-28, 1985.
Warranty/Conditions
Covance products may not be resold or modified for resale without prior written approval.