- 询价
- R&D
- 美国/中国
- xy-3324
- 2025年07月12日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液态/粉状
- 保存条件：
-20℃保存
- 克隆性：
多克隆
- 标记物：
见说明书
- 适应物种：
人，大鼠，小鼠，兔
- 宿主：
Goat，Rabbit，Mouse
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 靶点：
来电咨询
- CD20 B Cell Monoclonal Antibody (SIGNET)保存于运输说明：
- Store between 2-8°C.
- 详细信息：
-
Description 
Monoclonal Antibody against CD20 B Cell
(Formerly Signet Catalog Nos: 324-01, 324-16 L1 Predilute, 324-26 L2 Predilute, 324-36 LV Predilute)Intended Use ** In Vitro Diagnostic (IVD) ** Clone L26 Form Tissue Culture Supernatant (in 0.01M PBS + 0.1% NaN3 + 1% BSA) Host Mouse Species Reactivity Human IsoType CD20 B Cell Monoclonal Antibody (SIGNET) IgG2a Specificity The SIGNET anti CD20 monoclonal antibody recognizes the CD20 antigen, a molecule present on the majority of B cells present in peripheral blood and lymphoid tissue.
This antibody was generated using B cells derived from human tonsil.
Concentrated Format:
SIG-3324-1000 [1 mL]
Prediluted Formats:
SIG-3324-16 [6 mL] is ready to use with Peroxidase Anti- Peroxidase detection systems such as Signet’s PAP (SIG-32140).
SIG-3324-26 [6 mL] is ready-to-use with Biotin-based detection systems such as Signet’s USA Ultrastreptavidin Detection (SIG-32250).
SIG-3324-36 [6 mL] is ready-to-use with Biotin based detection systems on Ventana ES and Ventana NexES automated immunostainers.Uses CD20 B Cell Monoclonal Antibody (SIGNET) This antibody is effective in immunohistochemistry (IHC). Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. - IHC: ≥1:40 (concentrated format) with Biotin based detection systems such as Signet’s USA Ultrastreptavidin Detection (SIG-32250).
Tissue Sections: Formalin-fixed, paraffin-embedded tissues
Pretreatment: Not required
Incubation: 20-60 minutes at room temperature
Notes Positive tissue (human): Tonsil or lymph node Storage Store between 2-8°C. Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - IHC: ≥1:40 (concentrated format) with Biotin based detection systems such as Signet’s USA Ultrastreptavidin Detection (SIG-32250).
- CD20 B Cell Monoclonal Antibody (SIGNET)
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文献和实验Cell Kinetic Studies Using a Monoclonal Antibody to Bromodeoxyuridine
Cell kinetics is defined as the measurement of time parameters in biological systems. Traditionally, this has involved the use of radioactive precursors of DNA, such as tritiated thymidine (3 HTdR), and autoradiography to detect
attractive not only for studying various aspect of BCR signaling but also for the generation of human monoclonal antibody and the analysis of the human B cell repertoire. This chapter describes an efficient method to immortalize and clone human B cells
Monoclonal Antibody Production Protocol
(1)Cell Fusion and Selection Solutions: - IMDM - IMDM complete + 15% MCM. - PBS - PEG 50% ( w/v ): PEG 4000 ( 50% ) from Boehringer Manheim 1 243 268 - Aminopterin: Sigma, A 5159 (50X ) Prepare a T-150 flask with 170ml of IMDM
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