- ¥900 - 1890
- 钰博生物
- 见说明书
- 德国/美国/中国
- YBEB459Ge
- 2025年07月05日
14 年钻石会员
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
相关阅读
源叶生物 十万种现货试剂速发，品质稳、品类全，实验不再等！Takara 秋促来袭，提取+反转录+定量套装仅 1200 元abinScience 体内级抗体 全年锁价 低至 ￥1200阿拉丁 22万+ 科研级产品现货速发，品质严控、科研无忧- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 General，通用
卵白蛋白(OVA)检测试剂盒
检测范围 123.46-10000pg/mL 灵敏度 5pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长 2.5h 实验方法 竞争抑制法
规格 96T
卵白蛋白(OVA)检测试剂盒
ybB634Ra 大鼠骨骼肌肌动蛋白α1(ACTα1)ELISA试剂盒 ELISA Kit for Actin Alpha 1, Skeletal Muscle (ACTa1)
ybD419Ra 大鼠肌球蛋白重链6(MYH6)ELISA试剂盒 ELISA Kit for Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6)
ybF836Ra 大鼠转化受体电位阳离子通道亚家族M成员6(TRPM6)ELISA试剂盒 ELISA Kit for Transient ReHZptor Potential Cation Channel Subfamily M, Member 6 (TRPM6)
ybF837Ra 大鼠转化受体电位阳离子通道亚家族M成员7(TRPM7)ELISA试剂盒 ELISA Kit for Transient ReHZptor Potential Cation Channel Subfamily M, Member 7 (TRPM7)
ybB626Ra 大鼠白介素10受体α(IL10Rα)ELISA试剂盒 ELISA Kit for Interleukin 10 ReHZptor Alpha (IL10Ra)
ybB638Ra 大鼠白介素10受体β(IL10Rβ)ELISA试剂盒 ELISA Kit for Interleukin 10 ReHZptor Beta (IL10Rb)
ybB874Ra 大鼠凝血因子Ⅶ(F7)ELISA试剂盒 ELISA Kit for Coagulation Factor VII (F7)
ybB120Ra 大鼠精氨酸酶(Arg)ELISA试剂盒 ELISA Kit for ArginaHZ (Arg)
ybF416Ra 大鼠巢蛋白2(NID2)ELISA试剂盒 ELISA Kit for Nidogen 2 (NID2)
ybB404Ra 大鼠Thy1细胞表面抗原(Thy1)ELISA试剂盒 ELISA Kit for Thy1 HZll SurfaHZ Antigen (Thy1)
ybG515Ra 大鼠肥大细胞类糜蛋白酶1(CMA1)ELISA试剂盒 ELISA Kit for ChymaHZ 1, Mast HZll (CMA1)
ybD873Ra 大鼠胰岛素样蛋白3(INSL3)ELISA试剂盒 ELISA Kit for Insulin Like Protein 3 (INSL3)
ybG438Ra 大鼠钙结合蛋白(CALB)ELISA试剂盒 ELISA Kit for Calbindin (CALB)
ybB879Ra 大鼠载脂蛋白F(APOF)ELISA试剂盒 ELISA Kit for Apolipoprotein F (APOF)
ybA938Ra 大鼠足突蛋白(PDCN)ELISA试剂盒 ELISA Kit for Podocin (PDCN)
ybB267Ra 大鼠激肽原1(KNG1)ELISA试剂盒 ELISA Kit for Kininogen 1 (KNG1)
ybG501Ra 大鼠脂素1(LPIN1)ELISA试剂盒 ELISA Kit for Lipin 1 (LPIN1)
ybF320Ra 大鼠羧肽酶B1(CPB1)ELISA试剂盒 ELISA Kit for CarboxypeptidaHZ B1, Tissue (CPB1)
ybB897Ra 大鼠胰岛素降解酶(IDE)ELISA试剂盒 ELISA Kit for Insulin Degrading Enzyme (IDE)
卵白蛋白(OVA)检测试剂盒
检测范围 123.46-10000pg/mL 灵敏度 5pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长 2.5h 实验方法 竞争抑制法
规格 96T
卵白蛋白(OVA)检测试剂盒
ELISA Kit for Ovalbumin (OVA)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | General |
| Product No. | CEB459Ge |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Format | 96T |
| Assay length | 2.5 hours |
| Detection range | 123.46-10000pg/mL The standard curve concentrations used for the ELISA’s were 10000pg/mL, 3333.33pg/mL, 1111.11pg/mL, 370.37pg/mL, 123.46pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 5pg/mL. |
Specificity
卵白蛋白(OVA)检测试剂盒This assay has high sensitivity and excellent specificity for detection of Ovalbumin (OVA).
No significant cross-reactivity or interference between Ovalbumin (OVA) and analogues was observed.
No significant cross-reactivity or interference between Ovalbumin (OVA) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Ovalbumin (OVA) and the recovery rates were calculated by comparing the measured value to the expected amount of Ovalbumin (OVA) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 96-104 | 101 |
| EDTA plasma(n=5) | 83-97 | 89 |
| heparin plasma(n=5) | 83-103 | 92 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ovalbumin (OVA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ovalbumin (OVA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ovalbumin (OVA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ovalbumin (OVA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-98% | 79-101% | 89-101% | 81-88% |
| EDTA plasma(n=5) | 85-94% | 84-92% | 99-105% | 92-105% |
| heparin plasma(n=5) | 81-105% | 89-102% | 94-101% | 84-95% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37oC;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37oC;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
Test principle
卵白蛋白(OVA)检测试剂盒This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Ovalbumin (OVA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Ovalbumin (OVA) and unlabeled Ovalbumin (OVA) (Standards or samples) with the pre-coated antibody specific to Ovalbumin (OVA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Ovalbumin (OVA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Ovalbumin (OVA) in the sample.
ybE805Ra 大鼠N-甲基-D-天氡氨酸离子能谷氨酸受体1(GRIN1)ELISA试剂盒 ELISA Kit for Glutamate ReHZptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1)
ybB634Ra 大鼠骨骼肌肌动蛋白α1(ACTα1)ELISA试剂盒 ELISA Kit for Actin Alpha 1, Skeletal Muscle (ACTa1)
ybD419Ra 大鼠肌球蛋白重链6(MYH6)ELISA试剂盒 ELISA Kit for Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6)
ybF836Ra 大鼠转化受体电位阳离子通道亚家族M成员6(TRPM6)ELISA试剂盒 ELISA Kit for Transient ReHZptor Potential Cation Channel Subfamily M, Member 6 (TRPM6)
ybF837Ra 大鼠转化受体电位阳离子通道亚家族M成员7(TRPM7)ELISA试剂盒 ELISA Kit for Transient ReHZptor Potential Cation Channel Subfamily M, Member 7 (TRPM7)
ybB626Ra 大鼠白介素10受体α(IL10Rα)ELISA试剂盒 ELISA Kit for Interleukin 10 ReHZptor Alpha (IL10Ra)
ybB638Ra 大鼠白介素10受体β(IL10Rβ)ELISA试剂盒 ELISA Kit for Interleukin 10 ReHZptor Beta (IL10Rb)
ybB874Ra 大鼠凝血因子Ⅶ(F7)ELISA试剂盒 ELISA Kit for Coagulation Factor VII (F7)
ybB120Ra 大鼠精氨酸酶(Arg)ELISA试剂盒 ELISA Kit for ArginaHZ (Arg)
ybF416Ra 大鼠巢蛋白2(NID2)ELISA试剂盒 ELISA Kit for Nidogen 2 (NID2)
ybB404Ra 大鼠Thy1细胞表面抗原(Thy1)ELISA试剂盒 ELISA Kit for Thy1 HZll SurfaHZ Antigen (Thy1)
ybG515Ra 大鼠肥大细胞类糜蛋白酶1(CMA1)ELISA试剂盒 ELISA Kit for ChymaHZ 1, Mast HZll (CMA1)
ybD873Ra 大鼠胰岛素样蛋白3(INSL3)ELISA试剂盒 ELISA Kit for Insulin Like Protein 3 (INSL3)
ybG438Ra 大鼠钙结合蛋白(CALB)ELISA试剂盒 ELISA Kit for Calbindin (CALB)
ybB879Ra 大鼠载脂蛋白F(APOF)ELISA试剂盒 ELISA Kit for Apolipoprotein F (APOF)
ybA938Ra 大鼠足突蛋白(PDCN)ELISA试剂盒 ELISA Kit for Podocin (PDCN)
ybB267Ra 大鼠激肽原1(KNG1)ELISA试剂盒 ELISA Kit for Kininogen 1 (KNG1)
ybG501Ra 大鼠脂素1(LPIN1)ELISA试剂盒 ELISA Kit for Lipin 1 (LPIN1)
ybF320Ra 大鼠羧肽酶B1(CPB1)ELISA试剂盒 ELISA Kit for CarboxypeptidaHZ B1, Tissue (CPB1)
ybB897Ra 大鼠胰岛素降解酶(IDE)ELISA试剂盒 ELISA Kit for Insulin Degrading Enzyme (IDE)
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
小鼠卵白蛋白特异性IgG抗体(OVA-sIgG)酶联免疫分析（ELISA）
小鼠卵白蛋白 特异性IgG 抗体 ( OVA-sIgG ) 酶联免疫 分析（ ELISA ） 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定小鼠血清，血浆及相关液体样本中 卵白蛋白 特异性IgG抗体(OVA-sIgG) 含量。 实验原理 ： 本试剂盒应用 双抗原 夹心法测定 标本 中 小鼠卵白蛋白 特异性IgG 抗体 ( OVA-sIgG ) 水平。用纯化的 卵白蛋白 特异性IgG ( OVA-sIgG ) 抗原
6 ELISA SET操作说明中，则将生物素化的检测抗体和亲合素标记的HRP反应液预先混合后在加至洗过的反应孔中，相当于将前述流程中7、8、9步合在一起。类似地，作者用ELISA方法检测鸡卵白蛋白（OVA）免疫小鼠后血清内抗OVA抗体滴度时所用的简化程序如下：酶联板用OVA包被过夜→扣板→加入阻断缓冲液，室温下放置2小时→扣板→加入倍比稀释后的血清样本，室温下放置2小时→扣板→加入HRP标记的兔抗小鼠IgG抗体（Santa Cruz Biotechnology Inc，CA，USA），室温下放置1小时→洗
健康 Wistar 大鼠 雌雄不限 30 日龄 体重 60-80 克 ,随机分组 每组 10 只 依据分组不同分笼饲养 可以自由进食去卵白蛋白 不含鸡蛋 的特殊纯种饲料和水 动物房温度 19-25oC 湿度 55-65% 光照 10-12 小时/天 鼠笼每周清洗消毒两次1 致敏模型组 分别于第 1 天 第 8 天用新鲜配制的 OVA GradeV 1mg+ AI OH3 200mg+生理盐水 1ml 混悬液在大鼠两腹股沟 腹 前足跖 共 4 点作皮下注射每点 0.2ml 同时一点腹腔
技术资料暂无技术资料 索取技术资料
卵白蛋白(OVA)检测试剂盒
¥900 - 1890
