- ¥900 - 1890
- 钰博生物
- 见说明书
- 德国/美国/中国
- YBEA037Bo
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 Bos taurus; Bovine (Cattle，牛)
纤连蛋白(FN)检测试剂盒
检测范围 0.156-10ng/mL 灵敏度 0.065ng/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
纤连蛋白(FN)检测试剂盒
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ybA567Ra 大鼠S100钙结合蛋白B(S100B)ELISA试剂盒 ELISA Kit for S100 Calcium Binding Protein B (S100B)
ybA568Ra 大鼠S100钙结合蛋白A11(S100A11)ELISA试剂盒 ELISA Kit for S100 Calcium Binding Protein A11 (S100A11)
ybA571Ra 大鼠Ⅰ型胶原(COL1)ELISA试剂盒 ELISA Kit for Collagen Type I (COL1)
ybA572Ra 大鼠Ⅱ型胶原(COL2)ELISA试剂盒 ELISA Kit for Collagen Type II (COL2)
ybA573Ra 大鼠Ⅲ型前胶原氨基端原肽(PⅢNP)ELISA试剂盒 ELISA Kit for Procollagen III N-Terminal Propeptide (PIIINP)
ybA603Ra 大鼠载脂蛋白B100(APOB100)ELISA试剂盒 ELISA Kit for Apolipoprotein B100 (APOB100)
ybA604Ra 大鼠载脂蛋白A2(APOA2)ELISA试剂盒 ELISA Kit for Apolipoprotein A2 (APOA2)
ybA801Ra 大鼠碱性鞘磷脂磷酸二酯酶(Alk-SmaHZ)ELISA试剂盒 ELISA Kit for Alkaline SphingomyelinaHZ (Alk-SmaHZ)
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ybA799Ra 大鼠骨成型蛋白7(BMP7)ELISA试剂盒 ELISA Kit for Bone Morphogenetic Protein 7 (BMP7)
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纤连蛋白(FN)检测试剂盒
检测范围 0.156-10ng/mL 灵敏度 0.065ng/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
纤连蛋白(FN)检测试剂盒
ELISA Kit for Fibronectin (FN)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Bos taurus; Bovine (Cattle) |
| Product No. | SEA037Bo |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 0.065ng/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Fibronectin (FN).
No significant cross-reactivity or interference between Fibronectin (FN) and analogues was observed.
No significant cross-reactivity or interference between Fibronectin (FN) and analogues was observed.
Recovery
纤连蛋白(FN)检测试剂盒Matrices listed below were spiked with certain level of recombinant Fibronectin (FN) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibronectin (FN) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 78-93 | 87 |
| EDTA plasma(n=5) | 84-101 | 92 |
| heparin plasma(n=5) | 88-101 | 95 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibronectin (FN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibronectin (FN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibronectin (FN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibronectin (FN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 89-98% | 80-96% | 87-104% | 90-98% |
| EDTA plasma(n=5) | 78-90% | 87-96% | 84-94% | 91-99% |
| heparin plasma(n=5) | 89-103% | 78-95% | 80-94% | 86-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
纤连蛋白(FN)检测试剂盒The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibronectin (FN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibronectin (FN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibronectin (FN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibronectin (FN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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ybA835Ra 大鼠促肾上皮质激素释放激素(CRH)ELISA试剂盒 ELISA Kit for Corticotropin Releasing Hormone (CRH)
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ybA563Ra 大鼠白介素1β(IL1β)ELISA试剂盒 ELISA Kit for Interleukin 1 Beta (IL1b)
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ybA572Ra 大鼠Ⅱ型胶原(COL2)ELISA试剂盒 ELISA Kit for Collagen Type II (COL2)
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文献和实验相关实验
独体—基于纤连蛋白类型Ⅲ结构域框架的抗体模拟 Akiko Koide and Shohei Koide
片段的缺点，包括大尺寸、杂二聚特性（除单免疫球蛋白结构域外)、低构象稳定性和难于大规模生产。至今已发展出来的，这样的人工分子都是基于免疫球蛋白片段或者小单体蛋白。 我们发展了以纤连蛋白 Ⅲ 型结构域（FN3 ) 为分子框架的应用。纤连蛋白是一个大蛋白，在胞外基质生成和细胞间相互作用中起基本的作用。它由多次重复的 3 类（、Ⅱ 和 Ⅲ ) 小结构域组成 [3]。FN3 是广泛存在的结构域，在所有动物蛋白中，其出现率估计为 2% [4]。在人纤连蛋白中，有 15 个 FN3 的重复单位。我们的系统
细胞与细胞间基质的附着是细胞生存与增殖所必需的，这种附着主要由表达于各种组织细胞表面的整合素家族黏附分子来介导。除了p2组外，整合素分子识别的配体大都是细胞外基质的成分．包括纤连蛋白、层黏连蛋白、玻连蛋白和胶原等。细胞与基质的附着主要有以下两种情况：①间质细胞(以成纤维细抱为代表)的周围均与细胞外基质附着；②上皮细胞的周围仅部分与细胞外基质附着，而细胞侧面则与细胞发生附着．在这种情况下细胞黏附分子的分布存在着极性。细胞癌变过程往往伴随着这种极性的丧失。 3．黏附分子参与
白细胞分化抗原和黏附分子的胞膜外区结构 1．免疫球蛋白结构域和免疫球蛋白超家族 在白细胞分化抗原中，具有免疫球蛋白超家族(1gSF)结构域的分子约占1／3。IgSF结构域由90～110个氨基酸残基组成，可分为V样、C1和C2结构域。 2．Ⅲ型纤连蛋白(fibronectintypeⅢ，FN3)结构域 约由100个氨基酸残基组成，p―片层的折叠与IgSF和细胞因子受体结构域相似，但在序列上并无明显同源性。FN3通常有一个
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纤连蛋白(FN)检测试剂盒
¥900 - 1890
