- ¥900 - 1890
- 钰博生物
- 见说明书
- 德国/美国/中国
- YBEA049Ra
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 Rattus norvegicus (Rat，大鼠)
干扰素γ(IFNγ)检测试剂盒
检测范围 15.63-1000pg/mL 灵敏度 5.7pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
干扰素γ(IFNγ)检测试剂盒
ybA637Ra 大鼠氨基己糖苷酶Bβ(HEXβ)ELISA试剂盒 ELISA Kit for HexosaminidaHZ B Beta (HEXb)
ybA635Ra 大鼠补体因子H(CFH)ELISA试剂盒 ELISA Kit for Complement Factor H (CFH)
ybA634Ra 大鼠胰蛋白酶原激活肽(TAP)ELISA试剂盒 ELISA Kit for Trypsinogen Activation Peptide (TAP)
ybA638Ra 大鼠内脂素(VF)ELISA试剂盒 ELISA Kit for Visfatin (VF)
ybA675Ra 大鼠凝血因子Ⅴ(F5)ELISA试剂盒 ELISA Kit for Coagulation Factor V (F5)
ybA676Ra 大鼠凝血因子ⅩⅢ(F13)ELISA试剂盒 ELISA Kit for Coagulation Factor XIII (F13)
ybB842Ra 大鼠半胱氨酰白三烯受体2(CYSLTR2)ELISA试剂盒 ELISA Kit for Cysteinyl Leukotriene ReHZptor 2 (CYSLTR2)
ybB214Ra 大鼠天冬氨酸转氨酶(AST)ELISA试剂盒 ELISA Kit for Aspartate AminotransferaHZ (AST)
ybB176Ra 大鼠脑啡肽(ENK)ELISA试剂盒 ELISA Kit for Enkephalin (ENK)
ybA648Ra 大鼠Smad同源物7(Smad7)ELISA试剂盒 ELISA Kit for Mothers Against Decapentaplegic Homolog 7 (Smad7)
ybA647Ra 大鼠Smad同源物1(Smad1)ELISA试剂盒 ELISA Kit for Mothers Against Decapentaplegic Homolog 1 (Smad1)
ybA693Ra 大鼠27kDa热休克蛋白(HSPB1)ELISA试剂盒 ELISA Kit for Heat Shock 27kDa Protein 1 (HSPB1)
ybA639Ra 大鼠Ⅰ型胶原交联氨基端肽(NTXⅠ)ELISA试剂盒 ELISA Kit for Cross Linked N-Telopeptide Of Type I Collagen (NTXI)
ybA728Ra 大鼠环加氧酶1(PTGS1)ELISA试剂盒 ELISA Kit for Prostaglandin Endoperoxide SynthaHZ 1 (PTGS1)
ybA646Ra 大鼠骨形成蛋白6(BMP6)ELISA试剂盒 ELISA Kit for Bone Morphogenetic Protein 6 (BMP6)
ybA651Ra 大鼠透明质酸结合蛋白1(HABP1)ELISA试剂盒 ELISA Kit for Hyaluronan Binding Protein 1 (HABP1)
ybA929Ra 大鼠视黄醇结合蛋白4(RBP4)ELISA试剂盒 ELISA Kit for Retinol Binding Protein 4, Plasma (RBP4)
ybA653Ra 大鼠骨形成蛋白1(BMP1)ELISA试剂盒 ELISA Kit for Bone Morphogenetic Protein 1 (BMP1)
ybA991Ra 大鼠饥饿素(GHRL)ELISA试剂盒 ELISA Kit for Ghrelin (GHRL)
ybA987Ra 大鼠醛固酮合酶(ALDOS)ELISA试剂盒 ELISA Kit for Aldosterone SynthaHZ (ALDOS)
AEB264Ra 大鼠胰岛素自身抗体(IAA)ELISA试剂盒 ELISA Kit for Insulin Autoantibody (IAA)
ybA223Ra 大鼠白介素1受体拮抗剂(IL1RA)ELISA试剂盒 ELISA Kit for Interleukin 1 ReHZptor Antagonist (IL1RA)
ybA955Ra 大鼠Ⅰ型前胶原(PCⅠ)ELISA试剂盒 ELISA Kit for Procollagen I (PCI)
ybA449Ra 大鼠胱天蛋白酶7(CASP7)ELISA试剂盒 ELISA Kit for CaspaHZ 7 (CASP7)
干扰素γ(IFNγ)检测试剂盒
检测范围 15.63-1000pg/mL 灵敏度 5.7pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
干扰素γ(IFNγ)检测试剂盒
ELISA Kit for Interferon Gamma (IFNg)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Rattus norvegicus (Rat) |
| Product No. | SEA049Ra |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 15.63-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 5.7pg/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Interferon Gamma (IFNg).
No significant cross-reactivity or interference between Interferon Gamma (IFNg) and analogues was observed.
No significant cross-reactivity or interference between Interferon Gamma (IFNg) and analogues was observed.
Recovery
干扰素γ(IFNγ)检测试剂盒Matrices listed below were spiked with certain level of recombinant Interferon Gamma (IFNg) and the recovery rates were calculated by comparing the measured value to the expected amount of Interferon Gamma (IFNg) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 93-102 | 98 |
| EDTA plasma(n=5) | 78-101 | 93 |
| heparin plasma(n=5) | 99-105 | 102 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Gamma (IFNg) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Gamma (IFNg) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Gamma (IFNg) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interferon Gamma (IFNg) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-98% | 96-103% | 81-99% | 94-102% |
| EDTA plasma(n=5) | 78-96% | 83-94% | 88-103% | 79-97% |
| heparin plasma(n=5) | 91-99% | 97-105% | 80-90% | 94-102% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
干扰素γ(IFNγ)检测试剂盒The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Gamma (IFNg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Gamma (IFNg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Gamma (IFNg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Gamma (IFNg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ybA637Ra 大鼠氨基己糖苷酶Bβ(HEXβ)ELISA试剂盒 ELISA Kit for HexosaminidaHZ B Beta (HEXb)
ybA635Ra 大鼠补体因子H(CFH)ELISA试剂盒 ELISA Kit for Complement Factor H (CFH)
ybA634Ra 大鼠胰蛋白酶原激活肽(TAP)ELISA试剂盒 ELISA Kit for Trypsinogen Activation Peptide (TAP)
ybA638Ra 大鼠内脂素(VF)ELISA试剂盒 ELISA Kit for Visfatin (VF)
ybA675Ra 大鼠凝血因子Ⅴ(F5)ELISA试剂盒 ELISA Kit for Coagulation Factor V (F5)
ybA676Ra 大鼠凝血因子ⅩⅢ(F13)ELISA试剂盒 ELISA Kit for Coagulation Factor XIII (F13)
ybB842Ra 大鼠半胱氨酰白三烯受体2(CYSLTR2)ELISA试剂盒 ELISA Kit for Cysteinyl Leukotriene ReHZptor 2 (CYSLTR2)
ybB214Ra 大鼠天冬氨酸转氨酶(AST)ELISA试剂盒 ELISA Kit for Aspartate AminotransferaHZ (AST)
ybB176Ra 大鼠脑啡肽(ENK)ELISA试剂盒 ELISA Kit for Enkephalin (ENK)
ybA648Ra 大鼠Smad同源物7(Smad7)ELISA试剂盒 ELISA Kit for Mothers Against Decapentaplegic Homolog 7 (Smad7)
ybA647Ra 大鼠Smad同源物1(Smad1)ELISA试剂盒 ELISA Kit for Mothers Against Decapentaplegic Homolog 1 (Smad1)
ybA693Ra 大鼠27kDa热休克蛋白(HSPB1)ELISA试剂盒 ELISA Kit for Heat Shock 27kDa Protein 1 (HSPB1)
ybA639Ra 大鼠Ⅰ型胶原交联氨基端肽(NTXⅠ)ELISA试剂盒 ELISA Kit for Cross Linked N-Telopeptide Of Type I Collagen (NTXI)
ybA728Ra 大鼠环加氧酶1(PTGS1)ELISA试剂盒 ELISA Kit for Prostaglandin Endoperoxide SynthaHZ 1 (PTGS1)
ybA646Ra 大鼠骨形成蛋白6(BMP6)ELISA试剂盒 ELISA Kit for Bone Morphogenetic Protein 6 (BMP6)
ybA651Ra 大鼠透明质酸结合蛋白1(HABP1)ELISA试剂盒 ELISA Kit for Hyaluronan Binding Protein 1 (HABP1)
ybA929Ra 大鼠视黄醇结合蛋白4(RBP4)ELISA试剂盒 ELISA Kit for Retinol Binding Protein 4, Plasma (RBP4)
ybA653Ra 大鼠骨形成蛋白1(BMP1)ELISA试剂盒 ELISA Kit for Bone Morphogenetic Protein 1 (BMP1)
ybA991Ra 大鼠饥饿素(GHRL)ELISA试剂盒 ELISA Kit for Ghrelin (GHRL)
ybA987Ra 大鼠醛固酮合酶(ALDOS)ELISA试剂盒 ELISA Kit for Aldosterone SynthaHZ (ALDOS)
AEB264Ra 大鼠胰岛素自身抗体(IAA)ELISA试剂盒 ELISA Kit for Insulin Autoantibody (IAA)
ybA223Ra 大鼠白介素1受体拮抗剂(IL1RA)ELISA试剂盒 ELISA Kit for Interleukin 1 ReHZptor Antagonist (IL1RA)
ybA955Ra 大鼠Ⅰ型前胶原(PCⅠ)ELISA试剂盒 ELISA Kit for Procollagen I (PCI)
ybA449Ra 大鼠胱天蛋白酶7(CASP7)ELISA试剂盒 ELISA Kit for CaspaHZ 7 (CASP7)
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干扰素γ(IFNγ)检测试剂盒
¥900 - 1890
