适用生物 Homo sapiens (Human,人)
胰岛素样生长因子结合蛋白3(IGFBP3)检测试剂盒
检测范围 6.25-400ng/mL 灵敏度 2.52ng/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
胰岛素样生长因子结合蛋白3(IGFBP3)检测试剂盒
ELISA Kit for Insulin Like Growth Factor Binding Protein 3 (IGFBP3)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species |
Homo sapiens (Human) |
Product No. |
SEA054Hu |
Sample type |
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
Format |
96T |
Assay length |
4.5 hours |
Detection range |
6.25-400ng/mL The standard curve concentrations used for the ELISA’s were 400ng/mL, 200ng/mL, 100ng/mL, 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL |
Sensitivity |
The minimum detectable dose of this kit is typically less than 2.52ng/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor Binding Protein 3 (IGFBP3).
No significant cross-reactivity or interference between Insulin Like Growth Factor Binding Protein 3 (IGFBP3) and analogues was observed.
Recovery
胰岛素样生长因子结合蛋白3(IGFBP3)检测试剂盒Matrices listed below were spiked with certain level of recombinant Insulin Like Growth Factor Binding Protein 3 (IGFBP3) and the recovery rates were calculated by comparing the measured value to the expected amount of Insulin Like Growth Factor Binding Protein 3 (IGFBP3) in samples.
Matrix |
Recovery range (%) |
Average(%) |
serum(n=5) |
81-92 |
88 |
EDTA plasma(n=5) |
96-103 |
99 |
heparin plasma(n=5) |
88-97 |
92 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 3 (IGFBP3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 3 (IGFBP3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Insulin Like Growth Factor Binding Protein 3 (IGFBP3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
serum(n=5) |
83-101% |
91-99% |
90-98% |
95-103% |
EDTA plasma(n=5) |
85-98% |
96-105% |
78-103% |
80-97% |
heparin plasma(n=5) |
98-105% |
78-95% |
80-102% |
94-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents |
Quantity |
Reagents |
Quantity |
Pre-coated, ready to use 96-well strip plate |
1 |
Plate sealer for 96 wells |
4 |
Standard |
2 |
Standard Diluent |
1×20mL |
Detection Reagent A |
1×120µL |
Assay Diluent A |
1×12mL |
Detection Reagent B |
1×120µL |
Assay Diluent B |
1×12mL |
TMB Substrate |
1×9mL |
Stop Solution |
1×6mL |
Wash Buffer (30 × concentrate) |
1×20mL |
Instruction manual |
1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
胰岛素样生长因子结合蛋白3(IGFBP3)检测试剂盒The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor Binding Protein 3 (IGFBP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor Binding Protein 3 (IGFBP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor Binding Protein 3 (IGFBP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Like Growth Factor Binding Protein 3 (IGFBP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
yb-E10759 人红细胞生成素(EPO)ELISA试剂盒 Erythropoietin (EPO) ELISA kit
yb-E10760 人红细胞生成素受体(EPOR)ELISA试剂盒 Human erythropoietin receptor (EPOR) ELISA kit
yb-E10761 人β-微管蛋白(TUBB)ELISA试剂盒 Human β- tubulin (TUBB) ELISA Kit
yb-E10762 人前列腺素F2α(PGF2α)ELISA试剂盒 Human prostaglandin F2α (PGF2α) ELISA Kit
yb-E10763 人总前列腺特异抗原(tPSA)ELISA试剂盒 People total prostate specific antigen (tPSA) ELISA kit
yb-E10764 人抗人类免疫缺陷病毒抗体(HIV)ELISA试剂盒 Human antibodies against human immunodeficiency virus (HIV) ELISA Kit
yb-E10765 人解脲脲原体抗体(UU-Ab)ELISA试剂盒 Human antibody Ureaplasma urealyticum (UU-Ab) ELISA kit
yb-E10766 人复合前列腺特异性抗原(CPSA)ELISA试剂盒 Human prostate specific antigen complex (CPSA) ELISA kit
yb-E10767 人单纯疱疹病毒Ⅱ型抗体(HSVⅡ-Ab)ELISA试剂盒 Human herpes simplex virus type Ⅱ antibody (HSV Ⅱ-Ab) ELISA kit
yb-E10768 人细菌性阴道病(BV)ELISA试剂盒 Human bacterial vaginosis (BV) ELISA kit
yb-E10769 人单纯疱疹病毒Ⅰ型抗体(HSVⅠAb)ELISA试剂盒 Human herpes simplex virus type Ⅰ antibody (HSV Ⅰ Ab) ELISA kit
yb-E10770 人雌激素诱导蛋白PS2 ELISA试剂盒 Human estrogen -inducible protein PS2 ELISA kit
yb-E10771 人乳头状瘤病毒抗体IgM(HPV-IgM)ELISA试剂盒 Human papilloma virus antibody IgM (HPV-IgM) ELISA kit
yb-E10772 人乳头状瘤病毒抗体IgG(HPV-IgG)ELISA试剂盒 Human papilloma virus antibody IgG (HPV-IgG) ELISA Kit
yb-E10773 人单纯疱疹病毒抗原2(HSV-Ag2)ELISA试剂盒 Herpes Simplex Virus antigen 2 (HSV-Ag2) ELISA Kit
yb-E10774 人游离前列腺特异性抗原(fPSA)ELISA试剂盒 People free prostate specific antigen (fPSA) ELISA kit
yb-E10775 人前列腺特异性抗原(PSA)ELISA试剂盒 Human prostate specific antigen (PSA) ELISA kit
yb-E10776 人磷脂酰丝氨酸(PS)ELISA试剂盒 Human phosphatidylserine (PS) ELISA Kit
yb-E10777 人胆碱磷酸甘油酯(PC/CPG)ELISA试剂盒 Human choline phosphoglycerides (PC / CPG) ELISA Kit
yb-E10778 人总胆汁酸(TBA)ELISA试剂盒 People total bile acid (TBA) ELISA kit
yb-E10779 人载脂蛋白C3(Apo C3)ELISA试剂盒 Human apolipoprotein C3 (Apo C3) ELISA kit
yb-E10780 人胆固醇(CH)ELISA试剂盒 Human cholesterol (CH) ELISA kit
yb-E10781 人金属硫蛋白2(MT2)ELISA试剂盒 Human metallothionein 2 (MT2) ELISA kit
yb-E10782 人可溶性凝集素样氧化低密度脂蛋白受体1(sLOX-1)ELISA试剂盒 Human soluble lectin-like oxidized low-density lipoprotein receptor 1 (sLOX-1) ELISA Kit
yb-E10783 人3-硝基酪氨酸(3-NT)ELISA试剂盒 Man 3 - nitrotyrosine (3-NT) ELISA kit
yb-E10784 人糖胺聚糖(GAG)ELISA试剂盒 One glycosaminoglycan (GAG) ELISA Kit
yb-E10785 人半胱氨酰白三烯(CysLTs)ELISA试剂盒 People cysteinyl leukotrienes (CysLTs) ELISA kit
yb-E10786 人钙腔蛋白(CALU)ELISA试剂盒 Human calcium cavity protein (CALU) ELISA Kit
yb-E10787 人载脂蛋白A5(apo-A5)ELISA试剂盒 Human apolipoprotein A5 (apo-A5) ELISA kit
yb-E10788 人维生素D结合蛋白(DBP)ELISA试剂盒 Human vitamin D binding protein (DBP) ELISA Kit