- ¥900 - 1890
- 钰博生物
- 见说明书
- 德国/美国/中国
- YBEC918Hu
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 Homo sapiens (Human，人)
成纤维细胞生长因子21(FGF21)检测试剂盒
检测范围 7.81-500pg/mL 灵敏度 2.9pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
成纤维细胞生长因子21(FGF21)检测试剂盒
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yb-E12038 小鼠肿瘤标志物(CA724)ELISA试剂盒 Mouse tumor marker (CA724) ELISA Kit
yb-E12039 小鼠凝血因子Ⅱ(FⅡ)ELISA试剂盒 Clotting factor Ⅱ (F Ⅱ) ELISA kit
yb-E12040 小鼠内毒素(ET)ELISA试剂盒 Endotoxin (ET) ELISA kit for mouse
yb-E12041 小鼠心钠肽(ANP)ELISA试剂盒 Mouse atrial natriuretic peptide (ANP) ELISA kit
yb-E12042 小鼠过氧化物酶体增殖因子活化受体γ( PPAR-γ)ELISA试剂盒 Mouse peroxisome proliferator-activated receptor γ (PPAR-γ) ELISA kit
yb-E12043 小鼠毒性休克综合征毒素1(TSST-1)ELISA试剂盒 Toxin 1 (TSST-1) ELISA kit Mouse Toxic shock syndrome
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成纤维细胞生长因子21(FGF21)检测试剂盒
检测范围 7.81-500pg/mL 灵敏度 2.9pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
成纤维细胞生长因子21(FGF21)检测试剂盒
ELISA Kit for Fibroblast Growth Factor 21 (FGF21)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | SEC918Hu |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 7.81-500pg/mL The standard curve concentrations used for the ELISA’s were 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL, 7.81pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 2.9pg/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 21 (FGF21).
No significant cross-reactivity or interference between Fibroblast Growth Factor 21 (FGF21) and analogues was observed.
No significant cross-reactivity or interference between Fibroblast Growth Factor 21 (FGF21) and analogues was observed.
Recovery
成纤维细胞生长因子21(FGF21)检测试剂盒Matrices listed below were spiked with certain level of recombinant Fibroblast Growth Factor 21 (FGF21) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibroblast Growth Factor 21 (FGF21) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 81-94 | 91 |
| EDTA plasma(n=5) | 87-94 | 91 |
| heparin plasma(n=5) | 99-105 | 102 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 21 (FGF21) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 21 (FGF21) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibroblast Growth Factor 21 (FGF21) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibroblast Growth Factor 21 (FGF21) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-92% | 83-91% | 96-105% | 89-97% |
| EDTA plasma(n=5) | 80-102% | 89-97% | 81-91% | 80-89% |
| heparin plasma(n=5) | 90-101% | 97-105% | 81-98% | 80-103% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
成纤维细胞生长因子21(FGF21)检测试剂盒The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 21 (FGF21). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 21 (FGF21). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 21 (FGF21), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibroblast Growth Factor 21 (FGF21) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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yb-E12027 小鼠雌二醇受体(ER)ELISA试剂盒 Mouse estradiol receptor (ER) ELISA kit
yb-E12028 小鼠半乳糖6硫酸酯酶(Gal-6S)ELISA试剂盒 Sulfatase Galactose 6 mice (Gal-6S) ELISA Kit
yb-E12029 小鼠甲状旁腺激素相关蛋白(PTHrP)ELISA试剂盒 Mouse parathyroid hormone-related protein (PTHrP) ELISA kit
yb-E12030 小鼠苯丙氨酸(LPA)ELISA试剂盒 Mouse phenylalanine (LPA) ELISA Kit
yb-E12031 小鼠抗心磷脂抗体IgA(ACA-IgA)ELISA试剂盒 Mouse anti- cardiolipin antibodies IgA (ACA-IgA) ELISA kit
yb-E12032 小鼠抗心磷脂抗体IgM(ACA-IgM)ELISA试剂盒 Mouse anti- cardiolipin antibodies IgM (ACA-IgM) ELISA kit
yb-E12033 小鼠抗心磷脂抗体IgG(ACA-IgG)ELISA试剂盒 Mouse anti- cardiolipin antibodies IgG (ACA-IgG) ELISA Kit
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yb-E12044 小鼠补体1抑制物抗体(C1INH)ELISA试剂盒 A complement inhibitor mouse antibody (C1INH) ELISA Kit
yb-E12045 小鼠环孢素A(CsA)ELISA试剂盒 Mice cyclosporine A (CsA) ELISA kit
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成纤维细胞生长因子21(FGF21)检测试剂盒
¥900 - 1890
