- ¥900 - 1890
- 钰博生物
- 见说明书
- 德国/美国/中国
- YBEA532Hu
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 Homo sapiens (Human，人)
纤溶酶原激活物抑制因子1(PAI1)检测试剂盒
检测范围 15.63-1000pg/mL 灵敏度 6.5pg/mL
样本类型 Plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
纤溶酶原激活物抑制因子1(PAI1)检测试剂盒
yb-E12000 小鼠αN已酰氨基葡糖苷酶(αNAG)ELISA试剂盒 Mice αN glucosidase has amido (αNAG) ELISA Kit
yb-E12001 小鼠α甘露糖苷酶(α Manase)ELISA试剂盒 Mouse α -mannosidase (α Manase) ELISA Kit
yb-E12002 小鼠膜联蛋白Ⅴ(ANX-Ⅴ)ELISA试剂盒 Mice annexin Ⅴ (ANX-Ⅴ) ELISA kit
yb-E12003 小鼠抗内皮细胞抗体(AECA)ELISA试剂盒 Mouse anti- endothelial cell antibodies (AECA) ELISA kit
yb-E12004 小鼠α羟基丁酸脱氢酶(αHBDH)ELISA试剂盒 Mouse α hydroxybutyrate dehydrogenase (αHBDH) ELISA kit
yb-E12005 小鼠全段甲状旁腺素(i-PTH)ELISA试剂盒 Mice intact parathyroid hormone (i-PTH) ELISA kit
yb-E12006 小鼠中性粒细胞弹性蛋白酶(NE)ELISA试剂盒 Mouse neutrophil elastase (NE) ELISA Kit
yb-E12007 小鼠牛小肠碱性磷酸酶(CIAP)ELISA试剂盒 Mice calf intestinal alkaline phosphatase (CIAP) ELISA Kit
yb-E12008 小鼠α半乳糖基抗原(α-Gal)ELISA试剂盒 Mouse α galactosyl antigen (α-Gal) ELISA Kit
yb-E12009 小鼠β氨基己糖苷酶A(β-Hex A)ELISA试剂盒 Mouse β hexosaminidase A (β-Hex A) ELISA Kit
yb-E12010 小鼠肺表面活性物质相关蛋白A(SP-A)ELISA试剂盒 Mouse lung surface active substances associated protein A (SP-A) ELISA Kit
yb-E12011 小鼠克拉拉细胞蛋白(CC16)ELISA试剂盒 Mouse Clara cell protein (CC16) ELISA Kit
yb-E12012 小鼠β半乳糖苷酶(βGAL)ELISA试剂盒 Mouse β -galactosidase (βGAL) ELISA Kit
yb-E12013 小鼠肾上腺素(EPI)ELISA试剂盒 Mice epinephrine (EPI) ELISA Kit
yb-E12014 小鼠β甘露糖苷酶(β Manase)ELISA试剂盒 Mouse mannosidase β (β Manase) ELISA Kit
yb-E12015 小鼠髓过氧化物酶特异性抗中性粒细胞胞质抗体IgG(MPO-ANCA IgG)ELISA试剂盒 Mice myeloperoxidase specific anti -neutrophil cytoplasmic antibody IgG (MPO-ANCA IgG) ELISA Kit
yb-E12016 小鼠β内酰胺酶抑制剂(BLI)ELISA试剂盒 Mice β -lactamase inhibitor (BLI) ELISA Kit
yb-E12017 小鼠一氧化氮(NO)ELISA试剂盒 Mice Nitric oxide (NO) ELISA Kit
yb-E12018 小鼠β萘酚(β-Nph)ELISA试剂盒 Mouse β naphthol (β-Nph) ELISA Kit
yb-E12019 小鼠补体蛋白3(C3)ELISA试剂盒 Mouse complement protein 3 (C3) ELISA Kit
yb-E12020 小鼠叠氮胸苷(AZT)ELISA试剂盒 Mice azidothymidine (AZT) ELISA Kit
yb-E12021 小鼠β葡糖苷酶(β-glucosidase)ELISA试剂盒 Mouse β -glucosidase (β-glucosidase) ELISA Kit
yb-E12022 小鼠多巴胺(DA)ELISA试剂盒 Mice dopamine (DA) ELISA kit
纤溶酶原激活物抑制因子1(PAI1)检测试剂盒
检测范围 15.63-1000pg/mL 灵敏度 6.5pg/mL
样本类型 Plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
纤溶酶原激活物抑制因子1(PAI1)检测试剂盒
ELISA Kit for Plasminogen Activator Inhibitor 1 (PAI1)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | SEA532Hu |
| Sample type | Plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 15.63-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 6.5pg/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Plasminogen Activator Inhibitor 1 (PAI1).
No significant cross-reactivity or interference between Plasminogen Activator Inhibitor 1 (PAI1) and analogues was observed.
No significant cross-reactivity or interference between Plasminogen Activator Inhibitor 1 (PAI1) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Plasminogen Activator Inhibitor 1 (PAI1) and the recovery rates were calculated by comparing the measured value to the expected amount of Plasminogen Activator Inhibitor 1 (PAI1) in samples.
| Matrix | Recovery range (%) | Average(%) |
| sodium citrate plasma(n=5) | 80-101 | 91 |
Precision
纤溶酶原激活物抑制因子1(PAI1)检测试剂盒Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Plasminogen Activator Inhibitor 1 (PAI1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Plasminogen Activator Inhibitor 1 (PAI1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Plasminogen Activator Inhibitor 1 (PAI1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Plasminogen Activator Inhibitor 1 (PAI1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| sodium citrate plasma(n=5) | 91-99% | 79-90% | 91-99% | 90-99% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
纤溶酶原激活物抑制因子1(PAI1)检测试剂盒The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Plasminogen Activator Inhibitor 1 (PAI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Plasminogen Activator Inhibitor 1 (PAI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Plasminogen Activator Inhibitor 1 (PAI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Plasminogen Activator Inhibitor 1 (PAI1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
yb-E12000 小鼠αN已酰氨基葡糖苷酶(αNAG)ELISA试剂盒 Mice αN glucosidase has amido (αNAG) ELISA Kit
yb-E12001 小鼠α甘露糖苷酶(α Manase)ELISA试剂盒 Mouse α -mannosidase (α Manase) ELISA Kit
yb-E12002 小鼠膜联蛋白Ⅴ(ANX-Ⅴ)ELISA试剂盒 Mice annexin Ⅴ (ANX-Ⅴ) ELISA kit
yb-E12003 小鼠抗内皮细胞抗体(AECA)ELISA试剂盒 Mouse anti- endothelial cell antibodies (AECA) ELISA kit
yb-E12004 小鼠α羟基丁酸脱氢酶(αHBDH)ELISA试剂盒 Mouse α hydroxybutyrate dehydrogenase (αHBDH) ELISA kit
yb-E12005 小鼠全段甲状旁腺素(i-PTH)ELISA试剂盒 Mice intact parathyroid hormone (i-PTH) ELISA kit
yb-E12006 小鼠中性粒细胞弹性蛋白酶(NE)ELISA试剂盒 Mouse neutrophil elastase (NE) ELISA Kit
yb-E12007 小鼠牛小肠碱性磷酸酶(CIAP)ELISA试剂盒 Mice calf intestinal alkaline phosphatase (CIAP) ELISA Kit
yb-E12008 小鼠α半乳糖基抗原(α-Gal)ELISA试剂盒 Mouse α galactosyl antigen (α-Gal) ELISA Kit
yb-E12009 小鼠β氨基己糖苷酶A(β-Hex A)ELISA试剂盒 Mouse β hexosaminidase A (β-Hex A) ELISA Kit
yb-E12010 小鼠肺表面活性物质相关蛋白A(SP-A)ELISA试剂盒 Mouse lung surface active substances associated protein A (SP-A) ELISA Kit
yb-E12011 小鼠克拉拉细胞蛋白(CC16)ELISA试剂盒 Mouse Clara cell protein (CC16) ELISA Kit
yb-E12012 小鼠β半乳糖苷酶(βGAL)ELISA试剂盒 Mouse β -galactosidase (βGAL) ELISA Kit
yb-E12013 小鼠肾上腺素(EPI)ELISA试剂盒 Mice epinephrine (EPI) ELISA Kit
yb-E12014 小鼠β甘露糖苷酶(β Manase)ELISA试剂盒 Mouse mannosidase β (β Manase) ELISA Kit
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yb-E12017 小鼠一氧化氮(NO)ELISA试剂盒 Mice Nitric oxide (NO) ELISA Kit
yb-E12018 小鼠β萘酚(β-Nph)ELISA试剂盒 Mouse β naphthol (β-Nph) ELISA Kit
yb-E12019 小鼠补体蛋白3(C3)ELISA试剂盒 Mouse complement protein 3 (C3) ELISA Kit
yb-E12020 小鼠叠氮胸苷(AZT)ELISA试剂盒 Mice azidothymidine (AZT) ELISA Kit
yb-E12021 小鼠β葡糖苷酶(β-glucosidase)ELISA试剂盒 Mouse β -glucosidase (β-glucosidase) ELISA Kit
yb-E12022 小鼠多巴胺(DA)ELISA试剂盒 Mice dopamine (DA) ELISA kit
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纤溶酶原激活物抑制因子1(PAI1)检测试剂盒
¥900 - 1890
